An imbalance in interleukin-17-producing T and Foxp3+ regulatory T cells in women with idiopathic recurrent pregnancy loss
Authors: Lee, S.K.; Kim, J.Y.; Hur, S.E.; Kim, C.J.; Na, B.J.; Lee, M.; Gilman-Sachs, A.; Kwak-Kim, J.
Source: Human Reproduction, Volume 26, Number 11, 6 November 2011 , pp. 2964-2971(8)
Publisher: Oxford University Press
Abstract:BACKGROUNDT cells which produce interleukin (IL)-17 are involved in chronic inflammatory processes and regulatory T (Treg) cells are possibly the most important immune regulators. We aimed to investigate peripheral blood IL-17+ T and Foxp3+ Treg cells in women with idiopathic recurrent pregnancy loss (RPL).METHODSThe study design is a cross-sectional evaluation of Th1, Th2, IL-17+ T and Treg cells in women with idiopathic RPL (n = 42) and age-matched parous controls (n = 24). Flow cytometric analysis was performed to measure IL-17+ T and Foxp3+ Treg cells, and ratios of Th1/Th2 cells using anti-IL-17A and anti-Foxp3 antibodies, and monoclonal antibodies to tumor necrosis factor (TNF)-α, interferon (IFN)-γ and IL-10. Student's t-test and partial correlations were applied for statistical analysis.RESULTSTNF-α-/IL-10-producing CD3+CD4+ T cell ratio was higher in women with RPL than controls (P = 0.048). Levels of IL-17+ T cells (P = 0.021) and the IL-17+ T/CD4+Foxp3+ Treg cell ratio (P = 0.001) were increased, whereas Foxp3+ (P = 0.035), Foxp3low (P = 0.032) and CD4+Foxp3+ T cell (P = 0.037) levels were decreased in women with RPL, compared with controls. Levels of IL-17+ T cells were correlated with TNF-α-producing CD3+CD4+ T cells (r = 0.269, P = 0.033), and with ratios of TNF-α/IL-10 (r = 0.276, P = 0.027) and IFN-γ/IL-10 (r = 0.266, P = 0.035)-producing CD3+CD4+ cells. Furthermore, the ratio of IL-17+ T cells to CD4+Foxp3+ Treg cells showed a positive correlation with TNF-α-producing CD3+CD4+ T cells (P = 0.047) and IFN-γ-producing CD3+CD4+ T cells (P = 0.048) as well as a ratio of IFN-γ/IL-10-producing CD3+CD4+ T cells (P = 0.037).CONCLUSIONSEnhanced pro-inflammatory immune responses with suppressed immune regulation may be an important immune mechanism involved in RPL.
Document Type: Research article
Publication date: 2011-11-06
- Human Reproduction features full-length, peer-reviewed papers reporting original research, clinical case histories, as well as opinions and debates on topical issues. Papers published cover the scientific and medical aspects of reproductive physiology and pathology, endocrinology, andrology, gonad function, gametogenesis, fertilization, embryo development, implantation, pregnancy, genetics, genetic diagnosis, oncology, infectious disease, surgery, contraception, infertility treatment, psychology, ethics and social issues. The highest scientific and editorial standard is maintained throughout the journal along with a rapid rate of publication.