Effect of fetal or neonatal exposure to monobutyl phthalate (MBP) on testicular development and function in the marmoset
Authors: McKinnell, Chris; Mitchell, Rod T.; Walker, Marion; Morris, Keith; Kelnar, Chris J.H.; Wallace, W. Hamish; Sharpe, Richard M.
Source: Human Reproduction, Volume 24, Number 9, 28 September 2009 , pp. 2244-2254(11)
Publisher: Oxford University Press
Fetal exposure of male rats to some phthalates induces reproductive abnormalities, raising concerns for similar effects in humans. In order to address this in a more appropriate animal model, the aim of the present studies was to investigate the effect of fetal/neonatal exposure to monobutyl phthalate (MBP) in a non-human primate, the marmoset. In particular, to determine if exposure resulted in effects at birth, or in adulthood, similar to those in male rats, and whether there was evidence for induction of carcinoma-in-situ (CIS) or testicular germ cell tumours (TGCT).
Pregnant female marmosets were dosed from 715 weeks gestation with 500 mg/kg/day MBP and male offspring studied at birth (15 days; n 6) or in adulthood (n 5). In another study, newborn males (n 5 co-twins) were dosed with 500 mg/kg/day MBP for 14 days, commencing at 4 days of age.
Fetal exposure of marmosets to MBP did not affect gross testicular morphology, reproductive tract development or testosterone levels at birth, nor were germ cell number and proliferation, Sertoli cell number or germ:Sertoli cell ratio affected. In two of six MBP-exposed animals, unusual clusters of undifferentiated germ cells were found, but their significance is unclear. Neonatal MBP treatment did not affect germ cell numbers or differentiation. Fetal exposure to MBP did not affect testis size/morphology, germ cell numbers or fertility in adulthood. There was no evidence for CIS or TGCT.
Fetal exposure of marmosets to MBP does not measurably affect testis development/function or cause testicular dysgenesis, and no effects emerge by adulthood. Some effects on germ cell development were found, but these were inconsistent and of uncertain significance.
Document Type: Research Article
Publication date: 2009-09-28
- Human Reproduction features full-length, peer-reviewed papers reporting original research, clinical case histories, as well as opinions and debates on topical issues. Papers published cover the scientific and medical aspects of reproductive physiology and pathology, endocrinology, andrology, gonad function, gametogenesis, fertilization, embryo development, implantation, pregnancy, genetics, genetic diagnosis, oncology, infectious disease, surgery, contraception, infertility treatment, psychology, ethics and social issues. The highest scientific and editorial standard is maintained throughout the journal along with a rapid rate of publication.