Comparative protein-profile analysis of implanted versus non-implanted human blastocysts

Authors: Domnguez, Francisco; Gadea, Blanca; Esteban, Francisco J.; Horcajadas, Jose Antonio; Pellicer, Antonio; Simn, Carlos

Source: Human Reproduction, Volume 23, Number 9, 10 September 2008 , pp. 1993-2000(8)

Publisher: Oxford University Press

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Abstract:

BACKGROUND

New approaches for non-invasive embryo-quality assessment are among the major goals in Reproductive Medicine. We hypothesize that the detection of changes in the protein profile of the culture media in which blastocysts are cultured could be a potential indicator of the viability of the embryo and, thus, a useful tool for selecting the more appropriate blastocysts to be transferred.

METHODS

Using protein-array technology, we analysed the protein profile corresponding to 24 h conditioned media of blastocysts that implanted versus those that did not implant. A statistical approach was followed to compare each of these media versus a medium in the absence of blastocysts (control medium). In addition, a gene ontology functional analysisincluding those proteins showing a statistical difference among conditionswas performed, and a network with the predicted functional partners and corresponding relationships was obtained.

RESULTS

The soluble TNF receptor 1 and IL-10 increased significantly and MSP-, SCF, CXCL13, TRAILR3 and MIP-1 decreased significantly when the protein profile of the blastocyst culture medium was compared with the control medium. CXCL13 (BLC) and granulocyte-macrophage colonystimulating factor was also decreased significantly in the implanted blastocyst media compared with that in media from the non-implanted counterparts with a similar morphology. None of the proteins included in the array was increased significantly in the implanted blastocyst-conditioned media.

CONCLUSIONS

The differences identified in the protein profile of the culture media in the presence of implanted versus non-implanted blastocysts can be considered a new non-invasive approach in the search for new tools to diagnose blastocyst viability.

Keywords: implantation; secretome; implantome; protein array

Document Type: Research article

DOI: http://dx.doi.org/10.1093/humrep/den205

Publication date: 2008-09-10

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Human Reproduction features full-length, peer-reviewed papers reporting original research, clinical case histories, as well as opinions and debates on topical issues. Papers published cover the scientific and medical aspects of reproductive physiology and pathology, endocrinology, andrology, gonad function, gametogenesis, fertilization, embryo development, implantation, pregnancy, genetics, genetic diagnosis, oncology, infectious disease, surgery, contraception, infertility treatment, psychology, ethics and social issues. The highest scientific and editorial standard is maintained throughout the journal along with a rapid rate of publication.

Comparative protein-profile analysis of implanted versus non-implanted human blastocysts

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