Authors: Gremlich, S.; Fratta, S.; Rebellato, E.; Uras, R.; Reymondin, D.; Damnon, F.; Germond, M.; Gerber, S.

Source: Human Reproduction, Volume 23, Number 5, 17 May 2008 , pp. 1200-1206(7)

Publisher: Oxford University Press

Abstract:

BACKGROUND

Only 25 of IVF transfer cycles lead to a clinical pregnancy, calling for continued technical progress but also more in depth analysis of patients' individual characteristics. The interleukin-1 (IL-1) system and matrix metalloproteinases (MMPs) are strongly implicated in embryo implantation. The genes coding for IL-1Ra (gene symbol IL-1RN), IL-1, MMP2 and MMP9 bear functional polymorphisms. We analysed the maternal genetic profile at these polymorphic sites in IVF patients, to determine possible correlations with IVF outcome.

METHODS

One hundred and sixty women undergoing an IVF cycle were enrolled and a buccal smear was obtained. The presence of IL-1RN variable number of tandem repeats and IL-1B 3953, MMP2-1306 and MMP9-1562 single nucleotide substitutions were determined. Patients were divided into pregnancy failures (119), biochemical pregnancies (8) and clinical pregnancies (33).

RESULTS

There was a 40 decrease in IL-1RN2 allele frequency (P 0.024) and a 45 decrease in IL-1RN2 carrier status in the clinical pregnancy group as compared to the pregnancy failure group (P 0.017). This decrease was still statistically significant after a multivariate logistic regression analysis. The likelihood of a clinical pregnancy was decreased accordingly in IL-1RN2 carriers: odds ratio 0.349, 95 confidence interval 0.20.8, P 0.017. The IL-1B, MMP2 and MMP9 polymorphisms showed no correlation with IVF outcome.

CONCLUSIONS

IL-1RN2 allele carriage is associated with a poor prognosis of achieving a pregnancy after IVF.

Keywords: interleukin-1; receptor antagonist; polymorphism; IVF; clinical pregnancy

Document Type: Research article

DOI: http://dx.doi.org/10.1093/humrep/den034

Publication date: 2008-05-17

More about this publication?

• Human Reproduction features full-length, peer-reviewed papers reporting original research, clinical case histories, as well as opinions and debates on topical issues. Papers published cover the scientific and medical aspects of reproductive physiology and pathology, endocrinology, andrology, gonad function, gametogenesis, fertilization, embryo development, implantation, pregnancy, genetics, genetic diagnosis, oncology, infectious disease, surgery, contraception, infertility treatment, psychology, ethics and social issues. The highest scientific and editorial standard is maintained throughout the journal along with a rapid rate of publication.

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• In this Subject: Anatomy & Physiology ,  Obstetrics & Gynecology
• By this author: Gremlich, S. ;  Fratta, S. ;  Rebellato, E. ;  Uras, R. ;  Reymondin, D. ;  Damnon, F. ;  Germond, M. ;  Gerber, S.

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