Identification of differentially expressed markers in human follicular cells associated with competent oocytes
Authors: Hamel, Melanie; Dufort, Isabelle; Robert, Claude; Gravel, Catherine; Leveille, Marie-Claude; Leader, Arthur; Sirard, Marc-Andre
Source: Human Reproduction, Volume 23, Number 5, 17 May 2008 , pp. 1118-1127(10)
Publisher: Oxford University Press
The development of an accurate method for selection of high-quality embryos is essential to achieve high pregnancy rates with single embryo transfer in human IVF. The developmental competence of the oocyte is acquired during follicle maturation and strong communication also exists between the follicular cells (FCs) and the oocytes; thus oocyte developmental competence may be determined by markers expressed in the surrounding FCs.
From consenting patients (n 40), FCs were recovered on a per follicle basis by individual follicle puncture. Hybridization analyses using a custom-made complementary DNA microarray containing granulosa/cumulus expressed sequence tags (ESTs) from subtracted libraries and an Affymetrix GeneChip were performed to identify specific genes expressed in follicles leading to a pregnancy. The selected candidate genes were validated by quantitative-PCR (Q-PCR).
Subtractive libraries prepared from pooled samples representing pregnant versus non-pregnant patients produced 1694 ESTs. Hybridization data analysis discriminated 115 genes associated with competent follicles. Selected candidates were confirmed by Q-PCR: 3-beta-hydroxysteroid dehydrogenase 1 (P 0.0078), Ferredoxin 1 (P 0.0203), Serine (or cysteine) proteinase inhibitor clade E member 2 (P 0.0499), Cytochrome P450 aromatase (P 0.0359) and Cell division cycle 42 (P 0.0396).
Microarray technologies are useful to mine the transcriptome of FCs expressed in follicles associated with competent oocytes and could be used to improve embryo selection with the objective of successful single embryo transfer.
Document Type: Research Article
Publication date: 2008-05-17
- Human Reproduction features full-length, peer-reviewed papers reporting original research, clinical case histories, as well as opinions and debates on topical issues. Papers published cover the scientific and medical aspects of reproductive physiology and pathology, endocrinology, andrology, gonad function, gametogenesis, fertilization, embryo development, implantation, pregnancy, genetics, genetic diagnosis, oncology, infectious disease, surgery, contraception, infertility treatment, psychology, ethics and social issues. The highest scientific and editorial standard is maintained throughout the journal along with a rapid rate of publication.