Authors: Lisman, B.A.M.; van den Hoff, M.J.B.; Boer, K.; Bleker, O.P.; van Groningen, K.; Exalto, N.

Source: Human Reproduction, Volume 22, Number 8, 21 August 2007 , pp. 2254-2260(7)

Publisher: Oxford University Press

Abstract:

BACKGROUND

The aim of this study was to investigate normal chorionic villous vascularization using CD31 immunofluorescence and confocal laser scanning microscopy (CLSM) to elucidate the spatial arrangement in terms of connections between vessels and cords and their branching patterns compared to deficient chorionic villous vascularization in complicated pregnancies.

METHODS

A descriptive morphologic study using CLSM after CD31 immunofluorescence staining of placental biopsies from normal pregnancies (n 20), complete hydatidiform molar pregnancies (CHMs; n 3) and empty sacs (n 3), with a well documented gestational age (GA).

RESULTS

In this three-dimensional study, first trimester chorionic villi were occupied by a complex network of mainly cords with redundant connections as early as 5⁵ weeks GA. With increasing GA cords transform into vessels. From about 9 weeks GA onwards, vascular development is characterized by the presence of two large vessels located centrally and surrounded by and connected to a capillary network. In first trimester CHM and empty sacs, we observed a primitive network of mainly cords.

CONCLUSIONS

This first visualization of the spatio-temporal patterns of blood vessel formation in placental villi is characterized by the development of the vasculosyncytial membrane from a complex network of cords and can be regarded as the placental development before it becomes functional at the end of organogenesis.

Keywords: first trimester pregnancy; CD31; vasculogenesis; chorion villus; confocal laser scanning microscopy

Document Type: Research article

DOI: http://dx.doi.org/10.1093/humrep/dem143

Publication date: 2007-08-21

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• Human Reproduction features full-length, peer-reviewed papers reporting original research, clinical case histories, as well as opinions and debates on topical issues. Papers published cover the scientific and medical aspects of reproductive physiology and pathology, endocrinology, andrology, gonad function, gametogenesis, fertilization, embryo development, implantation, pregnancy, genetics, genetic diagnosis, oncology, infectious disease, surgery, contraception, infertility treatment, psychology, ethics and social issues. The highest scientific and editorial standard is maintained throughout the journal along with a rapid rate of publication.

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• In this Subject: Anatomy & Physiology ,  Obstetrics & Gynecology
• By this author: Lisman, B.A.M. ;  van den Hoff, M.J.B. ;  Boer, K. ;  Bleker, O.P. ;  van Groningen, K. ;  Exalto, N.

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