Concentration-dependent effects of a selective estrogen receptor modulator raloxifene on proliferation and apoptosis in human uterine leiomyoma cells cultured in vitro
Authors: Liu, Jin; Matsuo, Hiroya; Xu, Qin; Chen, Wei; Wang, Jiayin; Maruo, Takeshi
Source: Human Reproduction, Volume 22, Number 5, May 2007 , pp. 1253-1259(7)
Publisher: Oxford University Press
This study was conducted to elucidate the effects of raloxifene on proliferation and apoptosis in cultured human uterine leiomyoma cells.
The monolayer cultures were treated with graded concentrations (10−9, 10−8 and 10−7 M) of raloxifene and 10−7 M 17-estradiol (E
Compared with untreated cultures, the number of viable cultured cells, percentage of PCNA-positive cells and PCNA protein expression were significantly decreased by treatment with 10−9 M raloxifene, but increased by treatment with either 10−8 M or 10−7 M raloxifene. In contrast, the percentage of TUNEL-positive cells was significantly increased and Bcl-2 protein expression was significantly decreased by treatment with 10−9 M raloxifene, whereas they were not affected by treatment with either 10−8 or 10−7 M raloxifene.
In cultured leiomyoma cells, low concentration (10−9 M) of raloxifene may inhibit the growth of leiomyoma cells, whereas high concentrations (10−8 M, 10−7 M) of raloxifene may promote their growth.
Document Type: Research Article
Publication date: 2007-05-01
- Human Reproduction features full-length, peer-reviewed papers reporting original research, clinical case histories, as well as opinions and debates on topical issues. Papers published cover the scientific and medical aspects of reproductive physiology and pathology, endocrinology, andrology, gonad function, gametogenesis, fertilization, embryo development, implantation, pregnancy, genetics, genetic diagnosis, oncology, infectious disease, surgery, contraception, infertility treatment, psychology, ethics and social issues. The highest scientific and editorial standard is maintained throughout the journal along with a rapid rate of publication.