Human sperm chemotaxis: both the oocyte and its surrounding cumulus cells secrete sperm chemoattractants
Source: Human Reproduction, Volume 20, Number 3, 01 March 2005 , pp. 761-767(7)
Publisher: Oxford University Press
Abstract:BACKGROUND: Human sperm chemotaxis to pre-ovulatory follicular fluid is well established in vitro. However, it is not known whether the female's oocyte–cumulus complex secretes sperm chemoattractants subsequent to ovulation (for enabling sperm chemotaxis within the Fallopian tube) and, if so, which of these cell types—the oocyte or the cumulus oophorus—is the physiological origin of the secreted chemoattractant. METHODS: By employing a directionality-based chemotaxis assay, we examined whether media conditioned with either individual, mature (metaphase II) human oocytes or the surrounding cumulus cells attract human sperm by chemotaxis. RESULTS: We observed sperm chemotaxis to each of these media, suggesting that both the oocyte and the cumulus cells secrete sperm chemoattractants. CONCLUSIONS: These observations suggest that sperm chemoattractants are secreted not only prior to ovulation within the follicle, as earlier studies have demonstrated, but also after oocyte maturation outside the follicle, and that there are two chemoattractant origins: the mature oocyte and the surrounding cumulus cells.
Document Type: Research Article
Affiliations: 1: Department of Biological Chemistry, The Weizmann Institute of Science, 76100 Rehovot, 2: In-vitro Fertilization Unit, Department of Obstetrics and Gynecology, Barzilai Medical Center, Ben-Gurion University, 73806 Ashkelon, Israel and 3: Center for Cell and Molecular Biology, Faculty of Sciences, National University of Córdoba, Av. Vélez Sársfield 299, 5000–Córdoba, Argentina
Publication date: 2005-03-01
- Human Reproduction features full-length, peer-reviewed papers reporting original research, clinical case histories, as well as opinions and debates on topical issues. Papers published cover the scientific and medical aspects of reproductive physiology and pathology, endocrinology, andrology, gonad function, gametogenesis, fertilization, embryo development, implantation, pregnancy, genetics, genetic diagnosis, oncology, infectious disease, surgery, contraception, infertility treatment, psychology, ethics and social issues. The highest scientific and editorial standard is maintained throughout the journal along with a rapid rate of publication.