Restoration of fertility in infertile mice by transplantation of cryopreserved male germline stem cells
Source: Human Reproduction, Volume 18, Number 12, December 2003 , pp. 2660-2667(8)
Publisher: Oxford University Press
Abstract:BACKGROUND: The development of a spermatogonial transplantation technique has provided new possibilities for the treatment of male infertility. Previous studies have shown that spermatogonial stem cells could reinitiate spermatogenesis after cryopreservation and reintroduction into the seminiferous tubules of infertile recipient males, and this raised the possibility of banking frozen stem cells for male infertility treatment. It remains unknown, however, whether germ cells from freeze–thawed stem cells are fertile, leaving the possibility that the procedure compromises the integrity of the stem cells. METHODS AND RESULTS: Dissociated mouse testis cells were cryopreserved and transplanted into infertile recipient testes. The freeze–thawed testis cell populations contained higher concentrations of stem cells than fresh testis cell populations. Offspring were obtained from freeze–thawed stem cells transplanted into infertile males, and fertility restoration was more efficient in immature (5–10 days old) than in mature (6–12 weeks old) recipients. However, offspring were also obtained from infertile adult recipients using in‐vitro microinsemination. CONCLUSIONS: This first successful application of frozen stem cell technology in the production of offspring by spermatogonial transplantation suggests the superiority of immature recipients for clinical applications. Thus, the combination of cryopreservation and transplantation of stem cells is a promising approach to overcome male infertility.
Document Type: Research Article
Affiliations: 1: Horizontal Medical Research Organization, 2: The Institute of Physical and Chemical Research (RIKEN), Bioresource Center, Ibaraki, Japan 3: Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University and
Publication date: December 2003
- Human Reproduction features full-length, peer-reviewed papers reporting original research, clinical case histories, as well as opinions and debates on topical issues. Papers published cover the scientific and medical aspects of reproductive physiology and pathology, endocrinology, andrology, gonad function, gametogenesis, fertilization, embryo development, implantation, pregnancy, genetics, genetic diagnosis, oncology, infectious disease, surgery, contraception, infertility treatment, psychology, ethics and social issues. The highest scientific and editorial standard is maintained throughout the journal along with a rapid rate of publication.