Distribution of fetal erythroblasts enriched from maternal blood in multifetal pregnancies
Source: Human Reproduction, Volume 18, Number 9, September 2003 , pp. 1933-1936(4)
Publisher: Oxford University Press
Abstract:BACKGROUND: The aim of this study was to establish the frequency of fetal cells in the maternal blood of multifetal pregnancies and compare this figure with singleton pregnancies. METHODS: We obtained maternal blood from 31 pregnancies with 2–6 fetuses at 11–16 weeks gestation and from 50 normal singleton controls (11–14 weeks gestation). Fetal erythroblasts were isolated from maternal blood using triple density gradient separation and anti‐CD71 magnetic cell‐sorting techniques. The enriched erythroblasts were stained with Kleihauer–Giemsa and with fluorescent antibodies for the zeta (), epsilon () and gamma () globin chains. The percentage of fetal cells positive for each stain was calculated. Fluorescence in‐situ hybridization (FISH) for X and Y chromosomes was also performed. RESULTS: The percentage of erythroblasts enriched from maternal blood that stained positive for , and globin chains and with Kleihauer–Giemsa was significantly higher in the multifetal compared with singleton pregnancies. The median enriched percentage of positively stained erythroblasts was about three times higher in the twin than in singleton pregnancies (P < 0.0001), nearly twice as high in the triplet than in twin pregnancies (P < 0.01) and five times higher in the triplet than singleton pregnancies (P < 0.0001). FISH for Y chromosome confirmed the increase in fetal cell proportion in the multifetal pregnancies. CONCLUSIONS: These findings suggest that there is an increase in the physiological feto‐maternal cell trafficking in multifetal pregnancies compared with singleton pregnancies, which is likely to be due to the increased placental surface area and vasculature.
Document Type: Research Article
Affiliations: 1: Harris Birthright Research Centre For Fetal Medicine, 2: Department of Haematological Medicine and 3: Department of Molecular Medicine, King’s College London School of Medicine, Denmark Hill, London SE5 9RS, UK
Publication date: September 2003
- Human Reproduction features full-length, peer-reviewed papers reporting original research, clinical case histories, as well as opinions and debates on topical issues. Papers published cover the scientific and medical aspects of reproductive physiology and pathology, endocrinology, andrology, gonad function, gametogenesis, fertilization, embryo development, implantation, pregnancy, genetics, genetic diagnosis, oncology, infectious disease, surgery, contraception, infertility treatment, psychology, ethics and social issues. The highest scientific and editorial standard is maintained throughout the journal along with a rapid rate of publication.