Oestrogenic potencies of Zeranol, oestradiol, diethylstilboestrol, Bisphenol-A and genistein: implications for exposure assessment of potential endocrine disrupters
Authors: Leffers, Henrik; Næsby, Michael; Vendelbo, Brian; Skakkebæk, Niels E.; Jørgensen, Marianne
Source: Human Reproduction, Volume 16, Number 5, May 2001 , pp. 1037-1045(9)
Publisher: Oxford University Press
Abstract:We have compared the oestrogenic potency of the synthetic oestrogen Zeranol, used as a growth promoter in meat production, and five related compounds, with the potency of 17-oestradiol, diethylstilboestrol (DES), genistein, and Bisphenol-A. The potency was assayed by analysing differences in expression levels of endogenous oestrogen-regulated genes in human MCF7 cells, treated with different concentrations of the compounds. Zeranol, 17-oestradiol and DES were about equally potent, genistein was four to six orders of magnitude less potent than 17-oestradiol but an order of magnitude more potent than Bisphenol-A. There were gene specific differences, the PS2 and TGF3 genes were about equally sensitive to Zeranol, 17-oestradiol and DES whereas a down-regulation of MRG1/p35srj could be detected at fmol/l concentrations of Zeranol whereas 17-oestradiol was several orders of magnitude less potent. GST mu3 was sensitive to fmol/l concentrations of 17-oestradiol but much less sensitive to Zeranol and DES. The very high potency of Zeranol compared with other potential endocrine disrupters suggests that Zeranol intake from beef products could have greater impact on consumers than the amounts of the known or suspected endocrine disrupters that have been found in food. Since little data is available in man, there is an urgent need for reliable measurements of the concentration of Zeranol in human serum after ingestion of meat products from treated animals.
Document Type: Research Article
Affiliations: Department of Growth and Reproduction, Rigshospitalet, Copenhagen, Denmark
Publication date: May 2001
- Human Reproduction features full-length, peer-reviewed papers reporting original research, clinical case histories, as well as opinions and debates on topical issues. Papers published cover the scientific and medical aspects of reproductive physiology and pathology, endocrinology, andrology, gonad function, gametogenesis, fertilization, embryo development, implantation, pregnancy, genetics, genetic diagnosis, oncology, infectious disease, surgery, contraception, infertility treatment, psychology, ethics and social issues. The highest scientific and editorial standard is maintained throughout the journal along with a rapid rate of publication.