Infertility. A quantitative evaluation of α1, α4, αV and 3 endometrial integrins of fertile and unexplained infertile women during the menstrual cycle. A flow cytometric appraisal
Authors: Gonzalez, R.R.; Palomino, A.; Boric, A.; Vega, M.
Source: Human Reproduction, Volume 14, Number 10, October 1999 , pp. 2485-2492(8)
Publisher: Oxford University Press
Abstract:The expression of integrin molecules α11, α41 and αV3 within endometrial tissue has been proposed as a marker of uterine receptivity during the implantation window. The present investigation examines by flow cytometric analysis the concentrations of α1, α4, αV and 3 integrin subunits in endometrial stromal (ESC) and epithelial cells (EEC) in two groups of women throughout the menstrual cycle: normal fertile women (n = 27) and women with unexplained infertility (n = 26). Integrin concentrations in endometrial cells were calculated in relative fluorescence units against a negative cellular control. The assessment of integrin subunits detected the protein in ESC and EEC from the late proliferative to the late secretory phase. In both groups of women, the α1 was the highest integrin expressed in ESC and EEC throughout the menstrual cycle. All women exhibited low concentrations of α4-EEC at the time of the implantation window. Infertile women expressed lower concentrations of the α4-ESC during the proliferative and early secretory phase while lower concentrations of the α1-ESC were seen during the late secretory phase. Interestingly, the infertile women expressed lower concentrations of 3-EEC in the early, mid-secretory and late secretory phases (P < 0.05). Infertile women also expressed lower concentrations of α1-EEC and αV-EEC during the late secretory phase (P < 0.05). It can be concluded that the quantitative determination of 3-EEC by flow cytometry confirmed its potential feature as a marker of endometrial receptivity at the time of the implantation window. In addition, the defective expression of the α1-ESC found in the late secretory phase might be associated with the poor fertility outcome of women with unexplained infertility.
Document Type: Research Article
Affiliations: Institute of Maternal and Child Research (IDIMI), School Of Medicine, University Of Chile, `San Borja Arriaran' Clinical Hospital, and
Publication date: October 1999
- Human Reproduction features full-length, peer-reviewed papers reporting original research, clinical case histories, as well as opinions and debates on topical issues. Papers published cover the scientific and medical aspects of reproductive physiology and pathology, endocrinology, andrology, gonad function, gametogenesis, fertilization, embryo development, implantation, pregnancy, genetics, genetic diagnosis, oncology, infectious disease, surgery, contraception, infertility treatment, psychology, ethics and social issues. The highest scientific and editorial standard is maintained throughout the journal along with a rapid rate of publication.