Fertilization and early embryology. A prospective comparison of `in house' and commercially prepared Earle's balanced salt solution in human in-vitro fertilization
Authors: Karamalegos, C.; Bolton, V.N.
Source: Human Reproduction, Volume 14, Number 7, July 1999 , pp. 1842-1846(5)
Publisher: Oxford University Press
A prospective, randomized study was undertaken to compare the use of Earle's balanced salt solution (EBSS) prepared `in house' with that produced commercially, in 448 cycles of therapeutic in-vitro fertilization. Outcome was assessed in terms of fertilization and cleavage rates, embryo morphology, and implantation rates following embryo transfer. The only differences that were found between the two media in any of the outcome parameters were in the number of cycles with failed fertilization (1/218 in `in house' medium compared with 10/230 in commercially prepared medium; P = 0.0186), and in the rate at which embryos cleaved. Thus, while the median number of blastomeres per embryo was no different in the two groups at 46–49 h post insemination (three in embryos cultured in `in-house' medium, compared with four in those cultured in commercially prepared medium; P > 0.1), the number of embryos per cycle that had cleaved to the 4-cell stage by 46–49 h post insemination was significantly greater in the Medi-Cult than in the EBSS medium (P < 0.001).
Document Type: Original Article
Affiliations: Assisted Conception Unit, Department of Obstetrics and Gynaecology, King's College School of Medicine & Dentistry, Denmark Hill, London SE5 8RX, UK
Publication date: 1999-07-01
- Human Reproduction features full-length, peer-reviewed papers reporting original research, clinical case histories, as well as opinions and debates on topical issues. Papers published cover the scientific and medical aspects of reproductive physiology and pathology, endocrinology, andrology, gonad function, gametogenesis, fertilization, embryo development, implantation, pregnancy, genetics, genetic diagnosis, oncology, infectious disease, surgery, contraception, infertility treatment, psychology, ethics and social issues. The highest scientific and editorial standard is maintained throughout the journal along with a rapid rate of publication.