The development of smoothing-independent kinematic measures of capacitating human sperm movement
Authors: Mortimer, ST; Swan, MA
Source: Human Reproduction, Volume 14, Number 4, April 1999 , pp. 986-996(11)
Publisher: Oxford University Press
Abstract:The kinematic values which are determined relative to the average path by computer-aided sperm analysis (CASA) instruments are reliant upon the algorithm used for track smoothing, and therefore may differ depending upon the shape and complexity of the trajectories analysed. To overcome this potential source of error in the identification of particular trajectory patterns, kinematic values must be derived so that they are independent of the average path. In this study a number of novel kinematic measures were developed and tested for their ability to differentiate between hyperactivated and non-hyperactivated human spermatozoa. A definition for hyperactivation which relied solely upon smoothing-independent kinematic values was developed. This definition was tested on two groups of trajectories reconstructed at 60 Hz by a CASA instrument. An overall agreement of 99% between the classification of tracks as hyperactivated or non-hyperactivated was observed between the established 60 Hz definition validated for that particular CASA instrument and the new, smoothing-independent definition. Because the new values are only reliant (x, y) coordinates for their calculation, and do not require smoothing, it should not be difficult to incorporate them into existing CASA instruments.
Document Type: Research Article
Affiliations: Corresponding author
Publication date: 1999-04-01
- Human Reproduction features full-length, peer-reviewed papers reporting original research, clinical case histories, as well as opinions and debates on topical issues. Papers published cover the scientific and medical aspects of reproductive physiology and pathology, endocrinology, andrology, gonad function, gametogenesis, fertilization, embryo development, implantation, pregnancy, genetics, genetic diagnosis, oncology, infectious disease, surgery, contraception, infertility treatment, psychology, ethics and social issues. The highest scientific and editorial standard is maintained throughout the journal along with a rapid rate of publication.