Relaxin secretion by human granulosa cell culture is predictive of in-vitro fertilization-embryo transfer success
Authors: Stewart, DR; Vande Voort, CA
Source: Human Reproduction, Volume 14, Number 2, February 1999 , pp. 338-344(7)
Publisher: Oxford University Press
Abstract:We have developed a cell culture system for human luteinizing granulosa cells which supports the timely and dynamic secretion of oestrogen, progesterone and relaxin in patterns that mimic serum concentrations of these hormones during the luteal phase of the menstrual cycle. There was a wide variation in the amount of relaxin secreted by the cultured cells for the 69 patients studied. As relaxin production was generally maximal by day 10 of culture, comparisons were made at this time point. It was observed that most of the conceptions occurred in patients with higher relaxin secretion in vitro. All cycles with relaxin >800 pg/ml on day 10 had a term pregnancy while only 13% of cycles with relaxin <200 pg/ml had term pregnancies. A limited number of cycles from donor/recipient cycles did not show similar results. Steroid concentrations were not predictive of conception. These results demonstrated that in-vitro production of relaxin is predictive of implantation success in in-vitro fertilization (IVF)-embryo transfer cycles. This supports the hypothesis that relaxin may be involved in implantation and that lowered relaxin concentrations may be a partial cause of poor pregnancy rates after IVF.
Keywords:granulosa cell culture/infertility/in-vitro fertilization/pregnancy/relaxin
Document Type: Research Article
Affiliations: Division of Reproductive Biology, Department of Obstetrics and Gynecology, Institute of Toxicology and Environmental Health and
Publication date: 1999-02-01
- Human Reproduction features full-length, peer-reviewed papers reporting original research, clinical case histories, as well as opinions and debates on topical issues. Papers published cover the scientific and medical aspects of reproductive physiology and pathology, endocrinology, andrology, gonad function, gametogenesis, fertilization, embryo development, implantation, pregnancy, genetics, genetic diagnosis, oncology, infectious disease, surgery, contraception, infertility treatment, psychology, ethics and social issues. The highest scientific and editorial standard is maintained throughout the journal along with a rapid rate of publication.