Flow cytometric light scattering analysis, acrosome reaction, reactive oxygen species production and leucocyte contamination of semen preparation in prediction of fertilization rate in vitro
Source: Human Reproduction, Volume 13, Number 9, September 1998 , pp. 2568-2574(7)
Publisher: Oxford University Press
Abstract:The effects on fertilization of the morphology of spermatozoa, acrosome reaction, reactive oxygen species (ROS) production, leucocyte contamination and the light scattering in flow cytometry of semen preparation were investigated in 73 couples undergoing their first in-vitro fertilization treatment. All men had normal concentrations of spermatozoa with sufficient motility (50%) and yield (6 x 106) in the semen preparation on the day of oocyte retrieval. The light scattering properties of all cells present in the semen preparation, as assessed with flow cytometry, were correlated with fertilization. The proportion of metaphase I oocytes was also correlated with the fertilization rate of all collected oocytes and of metaphase II oocytes. ROS production, leucocyte contamination, spontaneous or calcium ionophore-stimulated acrosome reaction, percentage of normal morphology, and multiple anomalies index had no independent contribution.Key words:acrosome reaction/flow cytometry/in-vitro fertilization/reactive oxygen species/spermatozoa
Document Type: Research article
Affiliations: 1: Infertility Clinic, The Family Federation of Finland, Kalevankatu 16, 00100 Helsinki, Finland 2: The Haartman Institute, University of Helsinki, Haartmaninkatu 3, 00290 Helsinki, Finland
Publication date: 1998-09-01
- Human Reproduction features full-length, peer-reviewed papers reporting original research, clinical case histories, as well as opinions and debates on topical issues. Papers published cover the scientific and medical aspects of reproductive physiology and pathology, endocrinology, andrology, gonad function, gametogenesis, fertilization, embryo development, implantation, pregnancy, genetics, genetic diagnosis, oncology, infectious disease, surgery, contraception, infertility treatment, psychology, ethics and social issues. The highest scientific and editorial standard is maintained throughout the journal along with a rapid rate of publication.