Andrology: The presence of Müllerian inhibiting substance in human seminal plasma
Source: Human Reproduction, Volume 11, Number 10, October 1996 , pp. 2165-2169(5)
Publisher: Oxford University Press
Abstract:Müllerian inhibiting substance (MIS), produced by testicu-Iar Sertoli cells, is present in adult male serum. The first aim of this study was to determine if MIS is present in seminal plasma. Using an enzyme-linked immunosorbent assay (ELISA), we measured MIS concentrations in seminal plasma from 23 donors exhibiting normal (WHO criteria) sperm qualities, and 169 patients with subnormal sperm parameters. The second aim of this study was to examine a potential relationship between MIS and sperm motility. MIS concentrations in seminal plasma ranged from 0.5 to 3.6 ng/ml in donors and from 0.5 to 17.8 ng/ml in patients. Motility index (MI, mean ± SEM) for all patient samples was lower compared with donors (113.3 ± 3.2 and 1983 ± 13.5, P < 0.00001), while mean MIS concentration (± SEM) was higher (4.2 ± 03 and 1.4 ± 0.2, P < 0.0003). When the patients were stratified into Groups I (motility < 50%, n = 42) and D (motility >50%, n = 127), the MI (mean ± SEM) values were 623 ± 3.8 and 130.2 ± 2.7 respectively (P < 0.0001 for both compared with donors) and mean MIS concentrations (± SEM) were 5.4 ± 0.6 and 3.9 ± 03, respectively (P < 0.0001 and P < 0.001 compared with donors). The inverse relationship between MIS concentration in seminal plasma and motility index suggests that MIS may have a function in modulating motility.
Document Type: Research article
Affiliations: 1: <institution>Department of Surgery, University of Louisville</institution> <addr-line>Louisville, KY 40292</addr-line>, 2: <institution>Department of Surgery, University of Louisville</institution> <addr-line>Louisville, KY 40292</addr-line>, , 3: ,
Publication date: 1996-10-01
- Human Reproduction features full-length, peer-reviewed papers reporting original research, clinical case histories, as well as opinions and debates on topical issues. Papers published cover the scientific and medical aspects of reproductive physiology and pathology, endocrinology, andrology, gonad function, gametogenesis, fertilization, embryo development, implantation, pregnancy, genetics, genetic diagnosis, oncology, infectious disease, surgery, contraception, infertility treatment, psychology, ethics and social issues. The highest scientific and editorial standard is maintained throughout the journal along with a rapid rate of publication.