Transduction of 3prime-flanking sequences is common in L1 retrotransposition

Authors: Goodier, John L.; Ostertag, Eric M.; Kazazian, Haig H.

Source: Human Molecular Genetics, Volume 9, Number 4, 1 March 2000 , pp. 653-657(5)

Publisher: Oxford University Press

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Abstract:

Active LINE-1 (L1) elements possess the ability to transduce non-L1 DNA flanking their 3prime ends to new genomic locations. Occasionally, the 3prime end processing machinery may bypass the L1 polyadenylation signal and instead utilize a second downstream polyadeny- lation site. To determine the frequency of L1-mediated transduction in the human genome, we selected 66 previously uncharacterized L1 sequences from the GenBank database. Fifteen (23%) of these L1s had transposed flanking DNA with an average transduction length of 207 nucleotides. Since there are sim400 000 L1 elements, we estimate that insertion of transduced sequences alone may have enlarged the diploid human genome as much as 19 Mb or 0.6%. We also examined 24 full-length mouse L1s and found two long transduced sequences. Thus, L1 retrotransposition in vivo com- monly transduces sequence flanking the 3prime end of the element.

Document Type: Research article

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