An improved micropropagation protocol for maritime pine (Pinus pinaster Ait.) isolated cotyledons
Authors: lvarez, J. M.; Majada, J.; Ords, R. J.
Source: Forestry, Volume 82, Number 2, 27 April 2009 , pp. 175-184(10)
Publisher: Oxford University Press
Abstract:In this work an improved micropropagation protocol for Pinus pinaster Ait. using isolated cotyledons is presented. The variables studied during bud induction were embryo preculture, type and concentration of cytokinin and influence of illumination culture conditions. Shoot development and proliferation was obtained by transfer to hormone-free medium with activated charcoal. This protocol also describes a procedure for successful continuous multiplication using axillary shoots and brachyblasts formed during shoot development. Based on 86 per cent rooting success rate, at least 70 plantlets can be expected per seed after 16 weeks.
Document Type: Research Article
Publication date: 2009-04-27
- Forestry publishes refereed papers on all aspects of research, practice and policy that promote the sustainable development of forests, woodlands and trees. In considering suitability for publication attention is given to both the originality of contributions and their practical application. Preference is usually given to work undertaken in the temperate and/or boreal zones; only articles of exceptional merit from tropical zones will also be considered.