Plants and the Environment. Cloning of a cDNA encoding EIN3-like protein (DC-EIL1) and decrease in its mRNA level during senescence in carnation flower tissues

Authors: Waki K.; Shibuya K.; Yoshioka T.; Hashiba T.; Satoh S.

Source: Journal of Experimental Botany, Volume 52, Number 355, February 2001 , pp. 377-379(3)

Publisher: Oxford University Press

Abstract:

A cDNA clone encoding a putative EIN3-like protein (DC-EIL1) was obtained from total RNA isolated from senescing carnation (Dianthus caryophyllus L.) petals using RT-PCR and RACE techniques. The cDNA (2382 bp) contained an open reading frame of 1986 bp corresponding to 662 amino acids. The amino acid sequence of the N-terminal half of the protein, ranging from 80–300 amino acid residues, had 84% identity with that of the corresponding regions of Arabidopsis EIN3 and tobacco TEIL, although the overall identity was 49% and 52%, respectively. Northern blot analysis revealed that the amount of mRNA corresponding to DC-EIL1 decreased in flower tissues, especially in petals, during natural senescence and senescence induced by exogenously applied ethylene or ABA.

Keywords: Carnation; EIN3-like protein; ethylene signalling; flower senescence.

Document Type: Original article

Affiliations: Laboratory of Bioadaptation, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori-amamiyamachi 1-1, Aoba-ku, Sendai 981-8555, Japan

Publication date: 2001-02-01

• The Journal of Experimental Botany publishes high-quality primary research papers in the plant sciences. These papers cover a range of disciplines from molecular and cellular physiology and biochemistry through whole plant physiology to community physiology. Every issue of the Journal contains at least one 'Perspective' article. These are most commonly reviews of research areas, which are particularly exciting and important, topical or controversial. Opinion articles are also considered. In addition to 12 regular issues, at least one Special Issue is published each year. These are collection of articles derived from a specialised meeting or conference session. All papers are fully reviewed, and we will endeavour to complete the review process with all speed.

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