Plants and the Environment. Quantification of water transport in plants with NMR imaging
Authors: Scheenen, T.W.J.; van Dusschoten, D.; de Jager, P.A.; van As, H.
Source: Journal of Experimental Botany, Volume 51, Number 351, October 2000 , pp. 1751-1759(9)
Publisher: Oxford University Press
Abstract:A new nuclear magnetic resonance imaging (NMRi) method is described to calculate the characteristics of water transport in plant stems. Here, dynamic NMRi is used as a non-invasive technique to record the distribution of displacements of protons for each pixel in the NMR image. Using the NMR-signal of the stationary water in a reference tube for calibration, the following characteristics can be calculated per pixel without advance knowledge of the flow-profile in that pixel: the amount of stationary water, the amount of flowing water, the cross-sectional area of flow, the average linear flow velocity of the flowing water, and the volume flow. The accuracy of the method is demonstrated with a stem segment of a chrysanthemum flower by comparing the volume flow, measured with NMR, with the actual volumetric uptake, measured with a balance. NMR measurements corresponded to the balance uptake measurements with a rms error of 0.11 mg s-1 in a range of 0 to 1.8 mg s-1. Local changes in flow characteristics of individual voxels of a sample (e.g. intact plant) can be studied as a function of time and of any conceivable changes the sample experiences on a time-scale, longer than the measurement time of a complete set of pixel-propagators (17 min).
Document Type: Regular Paper
Affiliations: Department of Biomolecular Sciences, Laboratory of Molecular Physics, Wageningen University, Dreijenlaan 3, 6703 HA, Wageningen, The Netherlands
Publication date: 2000-10-01
- The Journal of Experimental Botany publishes high-quality primary research papers in the plant sciences. These papers cover a range of disciplines from molecular and cellular physiology and biochemistry through whole plant physiology to community physiology. Every issue of the Journal contains at least one 'Perspective' article. These are most commonly reviews of research areas, which are particularly exciting and important, topical or controversial. Opinion articles are also considered. In addition to 12 regular issues, at least one Special Issue is published each year. These are collection of articles derived from a specialised meeting or conference session. All papers are fully reviewed, and we will endeavour to complete the review process with all speed.