Cofactor requirement of ribosome-inactivating proteins from plants
Authors: Carnicelli, D; Brigotti, M; Alvergna, P; Pallanca, A; Sperti, S; Montanaro, L
Source: Journal of Experimental Botany, Volume 48, Number 313, August 1997 , pp. 1519-1523(5)
Publisher: Oxford University Press
Abstract:A large number of type 1 ribosome-inactivating proteins (RIPs) from plants (families of Caryophyllaceae, Cucurbitaceae, Euphorbiaceae, Phytolaccaceae, and Poaceae) were examined for their requirement for ATP and supernatant factors for full activity. A marked requirement was observed with agrostin among Caryophyllaceae, gelonin among Euphorbiacea, and with both barley RIP and tritin-S among Poaceae. The distribution of cofactor requirement in Phytolaccaceae discriminates leaf forms (cofactor-independent) from seed and root forms (cofactor-dependent). The results are discussed on the basis of the present knowledge on the tissue localization of RIPs and on the sensitivity of ribosomes to conspecific RIPs.
Keywords: Cofactors, ribosome-inactivating proteins, RNA-N-glycosidase, up-regulation.
Document Type: Research Article
Publication date: 1997-08-01
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