Evaluation of Cytotoxic Effects of Dichloromethane Extract of Guduchi (Tinospora cordifolia Miers ex Hook F & THOMS) on Cultured HeLa Cells

Authors: Jagetia, Ganesh Chandra; Rao, Shaival Kamalaksha

Source: Evidence-based Complementary and Alternative Medicine, Volume 3, Number 2, June 2006 , pp. 267-272(6)

Publisher: Oxford University Press

Buy & download fulltext article:

The full text is free.

View now:

PDF

Abstract:

Extracts of Tinospora cordifolia (TCE) have been shown to possess anti-tumor properties, but the mechanism of the anti-tumor function of TCE is poorly understood. This investigation elucidates the possible mechanism underlying the cytotoxic effects of dichlormethane extracts of TCE, after selecting optimal duration and concentration for treatment. HeLa cells were exposed to various concentrations of TCE, which has resulted in a concentration-dependent decline in the clonogenicity, glutathione-S-transferase (GST) activity and a concentration-dependent increase in lipid peroxidation (TBARS) with a peak at 4 h and lactate dehydrogenase (LDH) release with a peak at 2 h. Our results suggest that the cytotoxic effect of TCE may be due to lipid peroxidation and release of LDH and decline in GST.

Keywords: cytotoxicity; glutathione-S-transferase; Guduchi; HeLa; lactate dehydrogenase; lipid peroxidation

Document Type: Research article

DOI: http://dx.doi.org/10.1093/ecam/nel011

Publication date: 2006-06-01

More about this publication?

Evidence-based Complementary and Alternative Medicine (eCAM) is an international, peer-reviewed journal that seeks to understand the sources and to encourage rigorous research in this new, yet ancient world of complementary and alternative medicine.