IngentaConnect Fast Track Article: Evaluation of the presence of B-c...okines in chronic rhinosinusitis

Authors: M Patadia, J Dixon, D Conley, R Chandra, A Peters, L A Suh, A Kato, R Carter, K Harris, L Grammer, R Kern, R Schleimer

Abstract:

Background: B-cell responses may play a role in the pathogenesis of nasal polyposis via local IgA and IgE production and activation of eosinophils and mast cells. B-cell attracting chemokines may therefore have relevance in the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNPs)Methods: Polyp and inferior turbinate tissues were obtained from CRSwNPs, CRS without NPs (CRSsNPs), and control patients; ELISA and reverse-transcription polymerase chain reaction were used to detect levels of protein and mRNA for selected B-cell chemokines (B-cell attracting chemokine 1[CXCL13/BCA-1/BLC]), thymus expressed chemokine (CCL25/TECK), mucosae-associated epithelial chemokine (CCL28/MEC), stromal cell-derived factor-1alpha (CXCL12/SDF-1alpha), and selected chemokine receptor genes 9CXCR4, CXCR5, and CXCR7).Results: BCA-1 and SDF-1alpha protein levels were significantly increased in polyp tissue compared with turbinate tissue from CRSsNP patients andcontrols (p < 0.05 and p < 0.01, respectively). Differences in TECK and MEC were not significant. For mRNA, expression of BCA-1 was significantly up-regulated in polyp tissue and levels correlated with CD20 mRNA expression. Additionally, significant up-regulation of mRNA for the SDF-1alpha receptors CXCR7 and CXCR4 was detected in polyps, while there was a trend for up-regulation of the BCA-1 receptor CXCR5.Conclusion: Elevated levels of the BCA-1 and SDF-1alpha and their receptors may account for an increased presence of B cells and their products,contributing to eosinophilic inflammation in patients with CRSwNP

Evaluation of the presence of B-cell attractant chemokines in chronic rhinosinusitis