Peripheral blood and tissue T regulatory cells in chronic rhinosinusitis
Authors: Sharma, Shilpy; Watanabe, So; Sivam, Anita; Wang, JiangHong; Neuwirth, Samantha J.; Perez, Rosanne I.; De Tineo, Marcella; Baroody, Fuad M.; Naclerio, Robert M.; Pinto, Jayant M.
Source: American Journal of Rhinology & Allergy, Volume 26, Number 5, September/October 2012 , pp. 371-379(9)
Publisher: OceanSide Publications, Inc
The pathogenesis of chronic rhinosinusitis (CRS) has not been fully elucidated. Increased inflammatory cell infiltration and decreased numbers and/or impaired function of T regulatory cells (Tregs) have been reported. This study aimed to determine the role of Tregs in CRS in peripheral blood (PB) and sinus tissue.
Sinus tissue was obtained from 16 CRS subjects and 5 controls. PB from additional 16 CRS subjects and total 20 controls was obtained. Immunohistochemical analysis (CD3+, CD4+, CD8+, and Treg [CD4+-FoxP3+ and CD25+-FoxP3+] cells) of sinus tissue was performed. Percentage of PB Tregs (CD4+-CD25+-FoxP3+ cells) was analyzed by flow cytometry. Spontaneous and phytohemagglutinin (PHA)-induced release of cytokines (IL-6, IL-4, IL-10, interferon gamma, transforming growth factor [TGF] beta1, and TNF-alpha) from PB mononuclear cells (PBMCs) was determined.
PB flow cytometric analysis revealed a lower percentage of Tregs in subjects with CRS compared with healthy controls (p = 0.0003). Although no differences in the PB Treg counts were observed between the CRS subjects with nasal polyposis (CRSwNP) and without nasal polyposis (CRSsNP), immunohistochemical analysis performed on sinus tissue revealed a higher proportion of Tregs in CRSwNP subjects compared with CRSsNP (p < 0.05). Additionally, we failed to detect any Tregs from control sphenoid sinus tissue. Lower levels of regulatory cytokines (IL-10 and TGF-β1) and higher levels of proinflammatory cytokines (TNF-α and IL-6) were found from PBMCs from CRS subjects compared with controls (p < 0.05).
Our findings suggest that CRS subjects exhibit a decreased percentage of PB Tregs compared with normal controls. PBMCs from CRS subjects show a more proinflammatory and less regulatory phenotype.
Document Type: Research Article
Affiliations: Section of Otolaryngology-Head and Neck Surgery, Department of Surgery, The University of Chicago, Chicago, Illinois, USA
Publication date: September 1, 2012
- The American Journal of Rhinology & Allergy, is a peer reviewed, scientific publication committed to expanding knowledge and publishing the best clinical and basic research within the fields of Rhinology & Allergy. Its focus is to publish information which contributes to improved quality of care for patients with nasal and sinus disorders. Its primary readership consists of otolaryngologists, allergists, and plastic surgeons. Published material includes peer-reviewed original research, clinical trials and review articles.
Articles marked "F" offer free full text for personal noncommercial use only.
Previously published as American Journal of Rhinology, the journal is indexed in Thomson Reuters Web of Science and Science Citation Index, plus the National Library of Medicine's PubMed service.
- Editorial Board
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Information for Advertisers
- Reprint Requests
- ingentaconnect is not responsible for the content or availability of external websites
- In this: publication
- By this: publisher
- In this Subject: Allergy & Immunology , Ear, Nose & Throat
- By this author: Sharma, Shilpy ; Watanabe, So ; Sivam, Anita ; Wang, JiangHong ; Neuwirth, Samantha J. ; Perez, Rosanne I. ; De Tineo, Marcella ; Baroody, Fuad M. ; Naclerio, Robert M. ; Pinto, Jayant M.