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Soluble and nonsoluble protein assay for antigenic extracts from pollen and seeds of mustard (Brassica spp.)

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Abstract:

A study on heterogeneity in water-soluble and non–water-soluble protein profiles of different species of Brassica pollen, seeds, and industrial flour for efficient allergy detection and immunotherapy has been lacking in India. The purpose of this study was to examine heterogeneity in the protein profile of antigenic extracts of different species of pollen, seeds, and seed flour of Brassica. A comparison of water-soluble and non–water-soluble protein profiles of seed extracts was studied. Water-soluble and non–water-soluble proteins were extracted, concentration was estimated by Lowry's method, and biochemical characterization by sodium dodecyl sulfate–polyacrylamide gel electrophoresis was analyzed. Pollen from four species, four species of Brassica seeds comprising 25 varieties, and industrial seed flour were included for water-soluble studies, whereas four species of seeds were included for non–water-soluble protein extraction. Significant variation in protein content was observed among four different species of pollen, seeds, and industrial seed flour, respectively. No significant variation was observed in non–water-soluble extracts of four species or among water-soluble and non–water-soluble content of seeds. Heterogeneity in the protein profile of different species of pollen was not observed. However, variation in banding pattern of water-soluble as well as non–water-soluble protein extracts among four different species of seeds was observed. Interestingly, the industrial seed flour also showed a rich protein banding pattern. Variation in protein content as well as protein profile among different species of seeds of Brassica is recorded.

Keywords: Allergen characterization; Brassica; India; allergy; non-water soluble; pollen; protein; seeds; skin test; water soluble

Document Type: Research Article

DOI: https://doi.org/10.2500/aap.2007.28.3026

Affiliations: 1: Institute of Genomics and Integrative Biology, Delhi, India 2: Indian Agricultural Research Institute, Delhi, India 3: Delhi University, Delhi, India 4: From the Institute of Genomics and Integrative Biology, Delhi, India

Publication date: 2008-01-01

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  • Allergy and Asthma Proceedings is a peer reviewed publication dedicated to distributing timely scientific research regarding advancements in the knowledge and practice of allergy, asthma and immunology. Its primary readership consists of allergists and pulmonologists.

    The goal of the Proceedings is to publish articles with a predominantly clinical focus which directly impact quality of care for patients with allergic disease and asthma.

    Featured topics include asthma, rhinitis, sinusitis, food allergies, allergic skin diseases, diagnostic techniques, allergens, and treatment modalities. Published material includes peer-reviewed original research, clinical trials and review articles.

    Articles marked "F" offer free full text for personal noncommercial use only.

    The journal is indexed in Thomson Reuters Web of Science and Science Citation Index Expanded, plus the National Library of Medicine's PubMed service.
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