Increased expression of CD30 and CD57 molecules on CD4+ T cells from children with atopic asthma: A preliminary report

Authors: Rojas-Ramos, Enrique1; Garfias, Yonathan2; Jiménez-Martínez, María del Carmen2; Martínez-Jiménez, Norma1; Zenteno, Edgar3; Gorocica, Patricia4; Lascurain, Ricardo3

Source: Allergy and Asthma Proceedings, Volume 28, Number 6, November-December 2007 , pp. 659-666(8)

Publisher: OceanSide Publications, Inc

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Abstract:

T helper type 2 (Th2) cells play an important role in the onset and persistence of allergic airway inflammation. Consequently, many authors have attempted to identify cell surface markers associated with a Th2 phenotype. This work was aimed at correlating CD30 and CD57 expression on CD4+ T cells with interleukin (IL)-4 production in peripheral blood mononuclear cells (PBMCs) from allergic patients. PBMCs from 17 children with atopic asthma and 12 nonatopic healthy control children were analyzed. The CD28, CD30, CD40L, CD57, CD62L, CD69, IL-4, and IFN-gamma expressions on CD4+ T cells were determined by double immunofluorescence and flow cytometry in PBMCs ex vivo and after phorbol-12-myristate-13-acetate plus ionomycin (PMA/I) stimulation. An increased percentage of peripheral CD4+CD30+ T cells was observed in asthmatic patients (p < 0.001). In addition, the percentage of CD4+ T cells expressing IL-4, IFN-gamma, CD30, CD40L, CD57, or CD69 significantly increased (p < 0.01) after PMA/I stimulation, in asthmatic patients. The CD30 expression on CD4+ T cells from asthmatic patients, after stimulation, correlated with both IL-4 and IFN-gamma production, whereas CD57 expression only correlated with IL-4 production. These data suggest that the expression of CD30 and CD57 cell markers on T cells could reflect circulating effector T cell early activation in the allergic airway disease.

Keywords: Allergy; CD30; CD4; CD57; IL-4; T-lymphocyte; Th2; asthma; atopic; children

Document Type: Research Article

DOI: http://dx.doi.org/10.2500/aap.2007.28.3057

Affiliations: 1: Departamento de Inmunología Clínica y Alergia, Hospital Regional 1° de Octubre, ISSSTE, Mexico 2: Instituto Oftalmológico “Fundación Conde de Valenciana” AC, Mexico 3: Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico 4: Departamento de Bioquímica, Instituto Nacional de Enfermedades Respiratorias, Secretaría de Salud, Mexico

Publication date: November 1, 2007

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  • Allergy and Asthma Proceedings is a peer reviewed publication dedicated to distributing timely scientific research regarding advancements in the knowledge and practice of allergy, asthma and immunology. Its primary readership consists of allergists and pulmonologists.

    The goal of the Proceedings is to publish articles with a predominantly clinical focus which directly impact quality of care for patients with allergic disease and asthma.

    Featured topics include asthma, rhinitis, sinusitis, food allergies, allergic skin diseases, diagnostic techniques, allergens, and treatment modalities. Published material includes peer-reviewed original research, clinical trials and review articles.

    Articles marked "F" offer free full text for personal noncommercial use only.

    The journal is indexed in Thomson Reuters Web of Science and Science Citation Index Expanded, plus the National Library of Medicine's PubMed service.
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