If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email email@example.com
Mast cells (MCs) are important effector cells in mucosal defense and allergic inflammation. Lymphoid tissues such as tonsils and adenoids also have modest numbers of MCs. However, the role of MCs in lymphoid tissues is not well known. In this study, we showed the local distribution of MCs in tonsils from IgE-mediated allergic or nonallergic donors, studied their ultrastructure, and specified their surrounding cell types. Tonsils were obtained from IgE-mediated allergic or nonallergic donors suffering from chronic tonsillitis or hyperplastic tonsils. The localization and distribution of MCs and IgE binding to MCs were determined by immunohistochemistry using antibodies against tryptase, c-kit, and IgE. In addition, mast cell structure was examined using electron microscopy. In both allergic and nonallergic donors, MCs were distributed mainly in the interfollicular area and the perivascular area of connective tissue. We found that the total number of MCs in tonsils was almost the same in both donor groups. However, the number of MCs in the interfollicular area was significantly higher in nonallergic versus allergic donors. Moreover, the electron microscopy revealed that MCs localized in perivascular areas of connective tissue have scroll-type granules, and MCs in the interfollicular areas contained both particle and scroll-type granules. In addition, the MCs that were surrounded by CD4+ lymphocytes in the interfollicular area showed empty granules, whereas MCs in the perivascular area, not surround by CD4+, were intact. This implies that these MCs were degranulated, and this probably was caused by CD4+ cells. Taken together, these findings suggest that tonsillar MCs distribute differently in allergic and nonallergic donors, and that MCs in the interfollicular area might be activated by direct contact with CD4+ T cells.
Allergy and Asthma Proceedings is a peer reviewed publication dedicated to distributing timely scientific research regarding advancements in the knowledge and practice of allergy, asthma and immunology. Its primary readership consists of allergists and pulmonologists.
The goal of the Proceedings is to publish articles with a predominantly clinical focus which directly impact quality of care for patients with allergic disease and asthma.
Featured topics include asthma, rhinitis, sinusitis, food allergies, allergic skin diseases, diagnostic techniques, allergens, and treatment modalities. Published material includes peer-reviewed original research, clinical trials and review articles.
Articles marked "F" offer free full text for personal noncommercial use only.