Theophylline Inhibits IntegrinDependent Eosinophil Superoxide Production
Theophylline has been proposed as a drug that is able to reduce eosinophil activation in asthma. We tested the hypothesis that it can interfere with the integrin-mediated stimulation of eosinophil function. Eosinophils from healthy donors were triggered by monoclonal antibodies to 1- and 2-integrins in the presence of different concentrations of theophylline: 4.3 g/mL (2.4 × 10 −5M) 13 g/mL (7.2 × 10−5M) 26g/mL (1.4 × 10−4M), and 43 g/mL (2.4 × 10−4M), respectively. The level of activation was evaluated by assaying O−2 generation. A statistically significant inhibition (p < 0.05) of O−2 generation was observed with the different concentrations of theophylline when eosinophils were triggered via very late antigen 4 (VLA-4), lymphocyte function antigen 1 and the common 2-chain. No effect of theophylline on O−2 generation was observed in phorbol-myristate-acetate–stimulated eosinophils. These results suggest that theophylline can interfere with the eosinophil activation triggered by ligation of 1- and 2-integrins. This effect of theophylline possibly may play a relevant role in the inhibition of eosinophil infiltration and activation at the sites of allergic reactions.
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Document Type: Research Article
Publication date: 2005-01-01
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