Provider: Ingenta Connect Database: Ingenta Connect Content: application/x-research-info-systems TY - ABST AU - Ishizuka, Takao AU - Ishizuka, Tamotsu AU - Iwamae, Shigenori AU - Hisada, Takeshi AU - Okayama, Yoshimichi AU - Tsukagoshi, Hideo AU - Dobashi, Kunio AU - Mori, Masatomo TI - Interleukin-5 Messenger RNA Expression in Peripheral Blood Mononuclear Cells From Patients with Bronchial Asthma and Eosinophilia JO - Allergy and Asthma Proceedings PY - 2002-05-01T00:00:00/// VL - 23 IS - 3 SP - 175 EP - 179 N2 - Interleukin-5 (IL-5) plays an important role in allergic diseases accompanied by eosinophilia. We examined IL-5 messenger RNA (mRNA) expression in peripheral blood mononuclear cells (PBMC) isolated from 13 patients with bronchial asthma, 1 patient with chronic eosinophilic pneumonia, 2 patients with idiopathic eosinophilia, and 5 control subjects. IL-5 mRNA was expressed in PBMC from 2 of 13 patients with asthma, 1 patient with chronic eosinophilic pneumonia, and 1 of 2 patients with idiopathic eosinophilia. IL-5 mRNA expression was not detected in PBMC from control subjects. PBMC from a patient with chronic eosinophilic pneumonia expressed IL-5 mRNA spontaneously. Prednisolone treatment decreased his clinical symptoms and IL-5 mRNA expression. IL-5 mRNA expression preceded revival of the disease. These observations show that IL-5 plays a role in the condition, accompanied by eosinophilia, and IL-5 mRNA examination in PBMC by means of reverse transcription-polymerase chain reaction may be useful to predict the activity of eosinophilia. UR - https://www.ingentaconnect.com/content/ocean/aap/2002/00000023/00000003/art00002 ER -