Provider: Ingenta Connect
Database: Ingenta Connect
Content: application/x-research-info-systems
TY - ABST
AU - Klettke, Uwe
AU - Luck, Werner
AU - Wahn, Ulrich
AU - Niggemann, Bodo
TI - Platelet-Activating Factor Inhibits Ciliary Beat Frequency of Human Bronchial Epithelial Cells
JO - Allergy and Asthma Proceedings
PY - 1999-03-01T00:00:00///
VL - 20
IS - 2
SP - 115
EP - 118
N2 - The aim of our study was to investigate the effect of platelet-activating factor on human bronchial ciliary beat frequency (CBF) ex vivo. Brush biopsies were obtained from eight children (mean age 6.6 years) who underwent bronchoscopy for clinical reasons (i.e., foreign body aspiration, chronic cough, stridor). Immediate measurement of CBF by phase-contrast microscopy and photoelectric technique was performed before further treatment, and then on three subsamples: after adding platelet-activating factor (PAF) (10−5 M); after adding the inhibitor WEB 2086 (10−4 M); and after adding both PAF and WEB 2086. Addition of 10−5 M PAF reduced mean CBF by Hz to Hz (p < 0.05). The inhibitory effect of PAF was completely reversed by the addition of the PAF-inhibitor WEB 2086, whereas WEB 2086 alone did not significantly influence CBF. Optimum PAF concentration had been determined in previous dose–response experiments. Our data indicates that PAF involved in airway inflammation contributes to a decrease in mucociliary clearance in humans.
UR - https://www.ingentaconnect.com/content/ocean/aap/1999/00000020/00000002/art00006
M3 - doi:10.2500/108854199778612545
UR - https://doi.org/10.2500/108854199778612545
ER -