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Characterization of Latex Allergens and Correlation of Serum IgE/IgG Antibody Ratio with Clinical Symptoms

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Abstract:

Enzyme-linked immunosorbent assay (ELISA) was used to study latex-specific IgE and IgG. Levels of these immunoglobulins were correlated with latex allergy severity. Total protein content and relative immunoreactivity of 12 different brands of disposable gloves were compared. Specific latex proteins were extracted and characterized by immunoblotting. Blots revealed that the major latex allergen, reactive with both IgE and IgG, was a 34 KD component. Protein content varied among brands of gloves but correlated with immunoreactivity (R = 0.683; P < 0.017). The ratio of IgE/IgG correlated positively with symptom severity (R = 0.689; P < 0.001) and was useful to predict the severity of latex allergy.

Document Type: Research Article

DOI: http://dx.doi.org/10.2500/108854196779165058

Publication date: May 1, 1996

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  • Allergy and Asthma Proceedings is a peer reviewed publication dedicated to distributing timely scientific research regarding advancements in the knowledge and practice of allergy, asthma and immunology. Its primary readership consists of allergists and pulmonologists.

    The goal of the Proceedings is to publish articles with a predominantly clinical focus which directly impact quality of care for patients with allergic disease and asthma.

    Featured topics include asthma, rhinitis, sinusitis, food allergies, allergic skin diseases, diagnostic techniques, allergens, and treatment modalities. Published material includes peer-reviewed original research, clinical trials and review articles.

    Articles marked "F" offer free full text for personal noncommercial use only.

    The journal is indexed in Thomson Reuters Web of Science and Science Citation Index Expanded, plus the National Library of Medicine's PubMed service.
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