Solid Phase Enzyme-Immunoassays with Covalent Conjugates of Galactosidase
Beta galactosidase, from an i- constitutive strain of E. coli K12, possesses characteristics which are desirable for non-radioisotopic enzyme immunoassays. These characteristics include sensitivity of enzyme assay and availability of stable chromogenic and fluorogenic substrates. Galactosidase-antibody conjugates retain a high degree of enzymatic activity. A low tendency towards nonspecific absorption makes conjugates of galactosidase especially useful for solid phase immuno-assays. The application of galactosidase to various immuno-assays is reviewed. Data are presented which show the usefulness of galactosidase-conjugates for detection of murine lymphoblast cell membrane antigens. An application of galactosidase to detection of human total IgE in schistosomiasis is also demonstrated.
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Document Type: Research Article
Publication date: 1981-12-01
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