Solid Phase Enzyme-Immunoassays with Covalent Conjugates of Galactosidase
Abstract:Beta galactosidase, from an i- constitutive strain of E. coli K12, possesses characteristics which are desirable for non-radioisotopic enzyme immunoassays. These characteristics include sensitivity of enzyme assay and availability of stable chromogenic and fluorogenic substrates. Galactosidase-antibody conjugates retain a high degree of enzymatic activity. A low tendency towards nonspecific absorption makes conjugates of galactosidase especially useful for solid phase immuno-assays. The application of galactosidase to various immuno-assays is reviewed. Data are presented which show the usefulness of galactosidase-conjugates for detection of murine lymphoblast cell membrane antigens. An application of galactosidase to detection of human total IgE in schistosomiasis is also demonstrated.
Document Type: Research Article
Publication date: December 1, 1981
More about this publication?
- Allergy and Asthma Proceedings is a peer reviewed publication dedicated to distributing timely scientific research regarding advancements in the knowledge and practice of allergy, asthma and immunology. Its primary readership consists of allergists and pulmonologists.
The goal of the Proceedings is to publish articles with a predominantly clinical focus which directly impact quality of care for patients with allergic disease and asthma.
Featured topics include asthma, rhinitis, sinusitis, food allergies, allergic skin diseases, diagnostic techniques, allergens, and treatment modalities. Published material includes peer-reviewed original research, clinical trials and review articles.
Articles marked "F" offer free full text for personal noncommercial use only.
The journal is indexed in Thomson Reuters Web of Science and Science Citation Index Expanded, plus the National Library of Medicine's PubMed service.
- Editorial Board
- Information for Authors
- Submit a Paper
- Information for Advertisers
- Reprint Requests
- Ingenta Connect is not responsible for the content or availability of external websites