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Solid Phase Enzyme-Immunoassays with Covalent Conjugates of Galactosidase

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Beta galactosidase, from an i- constitutive strain of E. coli K12, possesses characteristics which are desirable for non-radioisotopic enzyme immunoassays. These characteristics include sensitivity of enzyme assay and availability of stable chromogenic and fluorogenic substrates. Galactosidase-antibody conjugates retain a high degree of enzymatic activity. A low tendency towards nonspecific absorption makes conjugates of galactosidase especially useful for solid phase immuno-assays. The application of galactosidase to various immuno-assays is reviewed. Data are presented which show the usefulness of galactosidase-conjugates for detection of murine lymphoblast cell membrane antigens. An application of galactosidase to detection of human total IgE in schistosomiasis is also demonstrated.
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Document Type: Research Article

Publication date: 1981-12-01

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  • Allergy and Asthma Proceedings is a peer reviewed publication dedicated to distributing timely scientific research regarding advancements in the knowledge and practice of allergy, asthma and immunology. Its primary readership consists of allergists and pulmonologists.

    The goal of the Proceedings is to publish articles with a predominantly clinical focus which directly impact quality of care for patients with allergic disease and asthma.

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    The journal is indexed in Thomson Reuters Web of Science and Science Citation Index Expanded, plus the National Library of Medicine's PubMed service.
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