If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

A high-density genetic linkage map of a black spruce (Picea mariana) × red spruce (Picea rubens) interspecific hybrid

$50.00 plus tax (Refund Policy)

Buy Article:

Abstract:

Genetic maps provide an important genomic resource of basic and applied significance. Spruce (Picea) has a very large genome size (between 0.85 × 1010 and 2.4 × 1010 bp; 8.5-24.0 pg/1C, a mean of 17.7 pg/1C ). We have constructed a near-saturated genetic linkage map for an interspecific backcross (BC1) hybrid of black spruce (BS; Picea mariana (Mill.) B.S.P.) and red spruce (RS; Picea rubens Sarg.), using selectively amplified microsatellite polymorphic loci (SAMPL) markers. A total of 2284 SAMPL markers were resolved using 31 SAMPL-MseI selective nucleotide primer combinations. Of these, 1216 SAMPL markers showing Mendelian segregation were mapped, whereas 1068 (46.8%) SAMPL fragments showed segregation distortion at α = 0.05. Maternal, paternal, and consensus maps consistently coalesced into 12 linkage groups, corresponding to the haploid chromosome number (1n = 1x = 12) of 12 in the genus Picea. The maternal BS map consisted of 814 markers distributed over 12 linkage groups, covering 1670 cM, with a mean map distance of 2.1 cM between adjacent markers. The paternal BS × RS map consisted of 773 markers distributed over 12 linkage groups, covering 1563 cM, with a mean map distance of 2.0 cM between adjacent markers. The consensus interspecific hybrid BC1 map consisted of 1216 markers distributed over 12 linkage groups, covering 1865 cM (98% genome coverage), with a mean map distance of 1.5 cM between adjacent markers. The genetic map reported here provides an important genomic resource in Picea, Pinaceae, and conifers.

Les cartes génétiques constituent des ressources importantes pour des travaux fondamentaux et appliqués. L’épinette (Picea) possède un très grand génome (0,85 × 1010 - 2,4 × 1010 pb; 8,5 - 24,0 pg/1C, 17,7 pg/1C en moyenne). Les auteurs ont produit une carte génétique presque saturée pour un rétrocroisement (BC1) interspécifique entre l’épinette noire (BS ; Picea mariana (Mill.) B.S.P.) et l’épinette rouge (RS ; Picea rubens Sarg.), en employant des marqueurs SAMPL (« selectively amplified microsatellite polymorphic loci »). Au total, 2284 marqueurs SAMPL ont été examinés à l’aide de 31 combinaisons d’amorces sélectives SAMPL-MseI. De ce nombre, 1216 marqueurs SAMPL présentant une ségrégation mendélienne ont été cartographiés, tandis 1068 (48,6 %) des fragments SAMPL affichaient une distorsion de la ségrégation au seuil de α = 0,05. Les cartes maternelle, paternelle et consensus étaient toutes constituées de 12 groupes de liaison, soit le nombre haploïde de chromosomes (1n = 1x = 12) chez le genre Picea. La carte maternelle BS comptait 814 marqueurs formant 12 groupes, de liaison s’étendant sur 1670 cM, pour une distance moyenne de 2,1 cM entre les marqueurs. La carte paternelle BS × RS totalisait 773 marqueurs sur 12 groupes, de liaison s’étendant sur 1563 cM, soit une moyenne d’un marqueur à tous les 2,0 cM. La carte consensus pour le rétrocroisement BC1 interspécifique comptait 1216 marqueurs sur 12 groupes, de liaison couvrant 1865 cM (98 % de couverture génomique), pour une distance moyenne de 1,5 cM entre les marqueurs. La carte génétique décrite dans ce travail représente une ressource génomique importante chez le genre Picea, les pinacées ainsi que les conifères.

Document Type: Research Article

Publication date: February 1, 2011

More about this publication?
  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Terms & Conditions
  • Sample Issue
  • Reprints & Permissions
  • ingentaconnect is not responsible for the content or availability of external websites
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more