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Development and characterization of microsatellite markers of the fungal plant pathogen Sclerotinia trifoliorum

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Sclerotinia trifoliorum is an important pathogen of forage legumes and some grain legumes. Attempts to study its population biology using microsatellite markers developed for Sclerotinia sclerotiorum and Sclerotinia subarctica resulted in no amplification or low levels of polymorphism. This study reports the development and characterization of 33 microsatellite loci developed from a microsatellite-enriched library of S. trifoliorum. Based on a population of 42 isolates of S. trifoliorum, these microsatellite markers are highly polymorphic, with a mean of 6.5 alleles per locus (range 3-12) and a mean expected heterozygosity of 0.63 (range 0.26-0.9). Based on locations of these marker sequences in the S. sclerotiorum genome, these microsatellite loci are dispersed throughout the genome. However, 50% (265 of 528) of pairwise comparisons of the 33 microsatellite loci had significant linkage disequilibrium, which could be explained by the mixed mating systems (homothallism and heterothallism) and clonal reproduction of S. trifoliorum. Thirty of the 33 loci were successfully applied to S. sclerotiorum, and 28 loci were polymorphic. However, only 10 loci are applicable to Sclerotinia minor and 1 locus to Sclerotinia homoeocarpa. These markers are therefore useful for population structure assessment, QTL mapping, and ecological analyses in S. trifoliorum and potentially in other Sclerotinia species.

Le Sclerotinia trifoliorum est un champignon pathogène important chez les légumineuses fourragères et quelques légumineuses à grains. Les tentatives visant à étudier sa biologie des populations au moyen de marqueurs microsatellites développés chez le S. sclerotiorum ou le S. subarctica ont produit soit aucune amplification ou un faible polymorphisme. Ce travail rapporte le développement et la caractérisation de 33 locus microsatellites à partir d’une banque génomique enrichie du S. trifoliorum. Sur la base d’une population de 42 isolats du S. trifoliorum, ces marqueurs se sont avérés très polymorphes et présentaient en moyenne 6,5 allèles par locus (de 3 à 12 allèles) et une hétérozygotie attendue moyenne de 0,63 (de 0,26 à 0,9). En fonction de la position de ces marqueurs dans le génome du S. sclerotiorum, ces locus seraient dispersés largement dans le génome. Cependant, 50 % (265/528) des paires de marqueurs examinées présentaient un déséquilibre de liaison significatif, observation qui pourrait s’expliquer par le mode de reproduction mixte (homothallique et hétérothallique) et la reproduction clonale chez le S. trifoliorum. Trente des 33 locus ont été employés avec succès chez le S. sclerotiorum et 28 de ceux-ci étaient polymorphes. Cependant, seuls 10 locus étaient utilisables chez le S. minor et un seul chez le S. homeocarpa. Ces marqueurs sont ainsi utiles pour l’étude de la structure des populations, la cartographie QTL et des analyses écologiques chez le S. trifoliorum et potentiellement d’autres espèces de Sclerotinia.

Document Type: Research Article

Publication date: June 1, 2010

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  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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