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Isolation and characterization of microsatellite loci for Euphorbia palustris (Euphorbiaceae)

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Abstract:

Swamp spurge (Euphorbia palustris, Euphorbiaceae) is a large perennial species of wet grassland and swamps. Its natural habitats are fragmented and isolated both naturally and owing to habitat destruction by human activity. Thus the species is endangered and legally protected in Germany. This report describes seven novel polymorphic microsatellite loci that will be helpful to characterize genetic variation and to analyze the population genetic structure and levels of gene flow within and among populations. All loci were amplified within one multiplex polymerase chain reaction for two populations, yielding between 3 and 13 alleles per locus and high levels of heterozygosity. Trans-species amplification is reported for four Euphorbia species.

L’euphorbe des marais (Euphorbia palustris, Euphorbiacées) est une espèce pérenne de grande taille qu’on retrouve dans les prairies humides et les marais. Ses habitats naturels sont fragmentés et isolés tant naturellement que suite à la destruction des habitats par les humains. Ainsi, cette espèce est menacée et bénéficie du statut d’espèce protégée en Allemagne. Dans ce travail, le développement de sept nouveaux marqueurs microsatellites est rapporté. Ceux-ci seront utiles pour caractériser la variation génétique et pour analyser la structure génétique au sein des populations de même que les flux géniques parmi et entre ces populations. Tous les locus ont été amplifiés lors d’une seule réaction en chaîne à la polymérase multiplexe pour deux populations, produisant entre 3 et 13 allèles par locus et des niveaux élevés d’hétérozygotie. La transportabilité de l’amplification sur d’autres espèces est rapportée pour quatre autres espèces du genre Euphorbia.

Document Type: Research Article

Publication date: 2009-12-01

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  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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