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Microsatellite-based evaluation of Ribes spp. germplasm

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Abstract:

There is a lack of published microsatellite data which characterizes Ribes spp. To address this, an initial study of simple sequence repeat (SSR) variation was undertaken in 41 cultivars belonging to four species of the genus Ribes to evaluate its genetic variability. The cultivars were collected in Piedmont, northwest Italy, together with one cultivar from Switzerland. Twenty SSRs were screened for amplification and polymorphism. Seven failed to amplify, and therefore the remaining 13 were selected and used to fingerprint all the cultivars. Microsatellite analysis resulted in the identification of 38 genotypes, suggesting the existence of possible clonal genotypes and synonyms. Among the cultivars analyzed, two tetraploid accessions were found. The evaluation of genetic variability in Ribes is of fundamental importance for future nutritional breeding programs and to preserve genetic resources, as cultivar characterization permits better management of plant collections.

Il n’y a pas de travaux publiés rapportant l’emploi de microsatellites pour caractériser les Ribes spp. Pour remédier à cette situation, une étude initiale de la variabilité génétique au sein de 41 cultivars appartenant à quatre espèces du genre Ribes a été réalisée à l’aide de microsatellites (SSR). Les cultivars provenaient du Piémont (nord-ouest de l’Italie) et, dans un cas, de la Suisse. Vingt microsatellites ont été examinés pour leur capacité à amplifier et à révéler du polymorphisme. Sept n’ont pas amplifié, alors les 13 autres ont été retenus et employés pour analyser tous les cultivars. L’analyse des microsatellites a permis d’identifier 38 génotypes, ce qui suggère l’existence possible de génotypes clonaux et de synonymes. Parmi les cultivars analysés, deux accessions tétraploïdes ont été trouvées. L’évaluation de la variabilité génétique chez le genre Ribes est d’importance fondamentale pour de futurs programmes de sélection nutritionnelle ainsi que pour préserver les ressources génétiques, la caractérisation des cultivars permettant une meilleure gestion des collections de ressources génétiques.

Document Type: Research Article

Publication date: October 1, 2009

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  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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nrc/gen/2009/00000052/00000010/art00003
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