Isolation and characterization of the rye Waxy gene

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Abstract:

Complete genomic and cDNA sequences of the Waxy gene encoding granule-bound starch synthase I (GBSSI) were isolated from the rye genome and characterized. The full-length rye Waxy genomic DNA and cDNA are 2767 bp and 1815 bp, respectively. The genomic sequence has 11 exons interrupted by 10 introns. The rye Waxy gene is GC-rich, with a higher GC frequency in the coding region, especially in the third position of the codons. Exon regions of the rye Waxy gene are more conserved than intron regions when compared with the homologous sequences of other cereals. The mature rye GBSSI proteins share more than 95% sequence identity with their homologs in wheat and barley. A phylogenetic tree based on sequence comparisons of available plant GBSSI proteins shows the evolutionary relationship among Waxy genes from rye and other plant genomes. The identification of the rye Waxy gene will enable the manipulation of starch metabolism in rye and triticale.

Des séquences génomiques et ADNc complètes du gène Waxy, lequel code pour l’amidon synthétase liée au grain I (GBSSI), ont été clonées et caractérisées chez le seigle. Les clones génomiques et ADNc du gène Waxy mesurent respectivement 2767 pb et 1815 pb. La séquence génomique compte 11 exons interrompus par 10 introns. Le gène Waxy du seigle est riche en GC et affiche une fréquence plus élevée en GC dans la région codante, particulièrement à l’endroit de la troisième position des codons. Les exons du gène Waxy du seigle sont plus conservés que les introns lorsque ces séquences sont comparées à leurs homologues chez d’autres céréales. Les protéines GBSSI matures présentent plus de 95 % d’identité avec leurs homologues chez le blé et l’orge. Un arbre phylogénétique comprenant les séquences de toutes les protéines GBSSI connues chez les plantes montre les relations évolutives entre les gènes Waxy du seigle et des autres plantes. L’identification du gène Waxy chez le seigle rendra possible la manipulation du métabolisme de l’amidon chez le seigle et le triticale.

Document Type: Research Article

Publication date: July 1, 2009

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  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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