A novel Rf gene controlling fertility restoration of Ogura male sterility by RNA processing of orf138 found in Japanese wild radish and its STS markers

$50.00 plus tax (Refund Policy)

Buy Article:

Abstract:

To reveal the molecular and genetic mechanism of fertility restoration in Ogura male sterility in Japanese wild radish (Raphanus sativus var. hortensis f. raphanistroides), we investigated fertility restoration of a plant that lacks the dominant type of orf687, a previously identified fertility restorer gene. A total of 100 F2 plants were made from the cross between a male-sterile strain with the Ogura cytoplasm, ‘MS-Gensuke’, and a Japanese wild radish plant. Segregation of pollen fertility in the F2 plants led us to assume that 2 dominant complementary genes controlled the fertility restoration of the plants. However, the fertility of 27 of 59 male-fertile plants was not completely restored, resulting in a group of plants with partial male fertility. Northern blot analysis of the CMS-associated gene orf138 indicated that one restorer allele (termed Rft) was involved in the processing of orf138 RNA. Rapid amplification of cDNA ends (RACE) and subsequent Northern blot analysis confirmed that the orf138 transcript lost a 5′ part of the coding region of the orf138 gene in the restored plants. The accumulation of ORF138 protein was significantly reduced by Rft, but trace amounts of the protein were recognized in both partially male-fertile and male-sterile plants with Rft. The relationship of pollen fertility and segregation of co-dominant sequence tagged site (STS) markers in the F2 generation suggested that the penetrance of Rft was so low that Rft needs suitable conditions to function sufficiently for the complete restoration of fertility.

Afin de mettre en évidence les mécanismes moléculaires et génétiques impliqués dans la restauration de la fertilité chez le système d’androstérilité Ogura du radis sauvage japonais (Raphanus sativus var. hortensis f. raphanistroides), les auteurs ont investigué la restauration de la fertilité chez une plante dépourvue du type dominant de l’orf687, un gène de restauration décrit antérieurement. Au total, 100 plantes F2 ont été obtenues en croisant une lignée androstérile ayant le cytoplasme Ogura, ‘MS-Gensuke’, et un plant de radis sauvage japonais. La ségrégation pour la fertilité du pollen chez les plantes F2 a permis d’avancer l’hypothèse que deux gènes complémentaires dominants contrôleraient la restauration de la fertilité chez les plantes. Cependant, la fertilité chez 27 des 59 plantes fertiles n’étaient pas complètement restaurée de telle sorte qu’un groupe à fertilité partielle a été observé. Des analyses northern du gène orf138, associé à l’androstérilité, a révélé qu’un des allèles restaurateurs (appelé Rft) était impliqué dans la maturation du transcrit de l’orf138. Une amplification des extrémités des ADNc (RACE) et une analyse northern subséquente ont confirmé que le transcrit de l’orf138 avait perdu une portion en 5′ de sa région codante chez les plants restaurés. L’accumulation de la protéine ORF138 était significativement réduite par Rft, bien que des traces de protéine aient été observées chez les plantes possédant Rft et qui étaient partiellement fertiles ou stériles. La relation entre la fertilité pollinique et la ségrégation de marqueurs STS (« sequence tagged sites ») codominants au sein de la génération F2 suggère que la pénétrance de Rft est si faible que Rft nécessite des conditions propices à un fonctionnement suffisant qui permet la restauration complète de la fertilité.

Document Type: Research Article

Publication date: June 1, 2009

More about this publication?
  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Terms & Conditions
  • Sample Issue
  • Reprints & Permissions
  • ingentaconnect is not responsible for the content or availability of external websites
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more