Authors: Terashima, Akihiro; Takumi, Shigeo

Source: Genome, Volume 52, Number 1, January 2009 , pp. 100-105(6)

Publisher: NRC Research Press

Abstract:

The wheat DREB2 homolog, WDREB2, encodes an EREBP/AP2-type transcription factor that activates downstream Cor/Lea genes and plays important roles in abiotic stress responses. WDREB2 expression is posttranscriptionally regulated by alternative splicing. In this study, the alternative splicing patterns of WDREB2 transcripts were compared among wheats of different ploidy levels. In diploid progenitors, alternative splicing rapidly produced functional forms from the WDREB2 transcripts and the levels of the nonfunctional form gradually decreased in response to drought stress; however, in hexaploid wheat lines, including both cultivars and synthetic lines, the nonfunctional form failed to decrease. The accumulation levels of the three spliced forms in synthetic allopolyploids were not equal to the sum of those in the parental lines. These results indicated that allopolyploidization during wheat polyploid evolution inhibited efficient alternative splicing of WDREB2 transcripts.

L'homologue de DREB2 chez le blé, WDREB2, code pour un facteur de transcription de type EREBP/AP2 qui active des gènes Cor/Lea en aval et qui joue des rôles importants dans les réponses aux stress abiotiques. L'expression de WDREB2 est régulée de manière post-transcriptionnelle via un épissage alternatif. Dans ce travail, les transcrits résultant de différents types d'épissage alternatif ont été comparés parmi des blés à différents niveaux de ploïdie. Chez les parents diploïdes, l'épissage alternatif a rapidement produit des formes fonctionnelles à partir des transcrits de WDREB2 et l'abondance des formes non-fonctionnelles a décliné graduellement en réponse à un stress hydrique. Cependant, chez des lignées hexaploïdes du blé, incluant tant des cultivars que des lignées synthétiques, l'abondance de la forme non-fonctionnelle n'a pas diminué. Chez les allopolyploïdes synthétiques, les niveaux d'accumulation des trois formes épissées n'égalaient pas la somme des niveaux dans les lignées parentales. Ces résultats indiquent que l'allopolyploïdisation au cours de l'évolution du blé polyploïde a inhibé l'épissage alternatif efficace des transcrits de WDREB2.

Document Type: Research article

Publication date: 2009-01-01

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• From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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