Authors: Fernandes, Thiago; Yuyama, Priscila M.; Moraes, Ana P.a.u.l.a.; Vanzela, André L.u..s.L.a.f.o.r.g.a.

Source: Genome, Volume 51, Number 9, September 2008 , pp. 772-777(6)

Publisher: NRC Research Press

Abstract:

Cestrum strigilatum (Solanaceae) is a South American shrub with B chromosomes. Bs show a univalent behavior when a single B is present, have non-Mendelian segregation, and are poor in genes and rich in repetitive DNA. In this study, the histone H3 at serine 10 (H3/Ser10) phosphorylation pattern was investigated during mitosis and meiosis of C. strigilatum collected from the wild and was compared in A and B chromosomes. The results revealed that H3/Ser10 phosphorylation of A chromosomes occurred only in the pericentromeric region in both mitosis and meiosis, whereas in the B univalent, phosphorylation appeared in almost the whole extent of the chromosome, except in the terminal portion of the long arm. In meiosis II, the phosphorylation of A chromosomes was similar to that in the first division of meiosis, but the Bs did not show H3/Ser10 phosphorylation. Our results suggest that phosphorylation at the pericentromeric region may be associated with chromosome motility during cell divisions and with the cohesion of B chromatids in a univalent structure in meiosis I.

Le Cestrum strigilatum (Solanacées) est un buisson d'Amérique du Sud qui possède des chromosomes B. Les chromosomes B forment des univalents lorsqu'un seul est présent, affichent une ségrégation non-mendélienne, sont pauvres en gènes et riches en ADN répété. Dans ce travail, la phosphorylation H3/Ser10 est examinée lors de la mitose et la méiose chez le C. strigilatum récolté dans la nature, et la présence de cette modification est comparée chez les chromosomes A et B. Les résultats montrent que la phosphorylation H3/Ser10 chez les chromosomes A était limitée aux régions péricentromériques tant lors de la mitose que de la méiose, tandis que chez l'univalent B, la phosphorylation était présente sur presque tout le chromosome à l'exception de la portion terminale du bras long. Lors de la méiose II, la phosphorylation des chromosomes A était semblable à celle observée lors de la première division de la méiose alors que les chromosomes B ne montraient pas de phosphorylation H3/Ser10. Ces résultats suggèrent que la phosphorylation des régions péricentromériques serait possiblement associée avec la mobilité des chromosomes au cours des divisions cellulaires ainsi qu'avec la cohésion des chromatides B au sein d'un univalent à la méiose I.

Document Type: Research article

Publication date: 2008-09-01

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• From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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