Authors: Poncet, Valérie; Dufour, Magali; Hamon, Perla; Hamon, Serge; de Kochko, Alexandre; Leroy, Thierry

Source: Genome, Volume 50, Number 12, December 2007 , pp. 1156-1161(6)

Publisher: NRC Research Press

Abstract:

Of the 103 accepted Coffea species, 70% are threatened with extinction but only a few of them have been studied. A set of 40 polymorphic microsatellite markers was developed using a GA/GT-enriched Coffea canephora genomic library. Amplification of these markers was tested in accessions of C. heterocalyx (a Critically Endangered species) and C. pseudozanguebariae (a Vulnerable species) belonging to different African geographical clades. All microsatellites were polymorphic in C. canephora, with a mean allele number per polymorphic locus of more than 3 (at least 9 genotypes were tested). Observed and expected heterozygosities calculated for C. canephora and C. pseudozanguebariae varied from 0.10 to 0.91 and from 0.20 to 0.77, respectively. In total, 38 primer pairs (95%) were amplified in C. heterocalyx and C.pseudozanguebariae, indicating their high level of transferability across the genus Coffea. This large marker set will be useful for more extensive genetic studies of threatened Coffea species.

Soixante-dix pour cent des 103 espèces de Coffea actuellement identifiées sont menacées d'extinction, mais peu d'entre elles sont étudiées. Un jeu de 40 marqueurs microsatellites polymorphes a été développé en utilisant une banque génomique de Coffea canephora enrichie en GA/GT. L'amplification de ces marqueurs a été testée sur des accessions de C. heterocalyx (une espèce déclarée «Gravement Menacée») et C. pseudozanguebariae (une espèce déclarée «Vulnérable»), appartenant à différents clades géographiques Africains. Tous les microsatellites sont polymorphes chez C. canephora, avec un nombre moyen d'allèles par locus de plus de 3 (sur un minimum de 9génotypes testés). Les hétérozygoties observées et attendues, calculées pour les espèces C. canephora et C. pseudozanguebariae varient respectivement entre 0,10 et 0,91; et entre 0,20 et 0,77. Au total, 95 % des paires d'amorces ont permis une amplification chez C.heterocalyx et C. pseudozanguebariae, indiquant un fort taux de transfert au sein du genre Coffea. Ce large jeu de marqueurs sera utile pour des études génétiques approfondies des espèces menacées de Coffea.

Document Type: Research article

Publication date: 2007-12-01

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• From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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