Authors: Vorwerk, S.; Forneck, A.

Source: Genome, Volume 50, Number 7, July 2007 , pp. 660-667(8)

Publisher: NRC Research Press

Abstract:

Two AFLP fingerprinting methods were employed to estimate the potential of AFLP fingerprints for the detection of genetic diversity within single founder lineages of grape phylloxera (Daktulosphaira vitifoliae Fitch). Eight clonal lineages, reared under controlled conditions in a greenhouse and reproducing asexually throughout a minimum of 15 generations, were monitored and mutations were scored as polymorphisms between the founder individual and individuals of succeeding generations. Genetic variation was detected within all lineages, from early generations on. Six to 15 polymorphic loci (from a total of 141 loci) were detected within the lineages, making up 4.3% of the total amount of genetic variation. The presence of contaminating extra-genomic sequences (e.g., viral material, bacteria, or ingested chloroplast DNA) was excluded as a source of intraclonal variation. Sequencing of 37 selected polymorphic bands confirmed their origin in mostly noncoding regions of the grape phylloxera genome. AFLP techniques were revealed to be powerful for the identification of reproducible banding patterns within clonal lineages.

Deux méthodes d'empreintes génétiques AFLP ont été employées afin de mesurer le potentiel des AFLP pour la détection de la diversité génétique présente au sein de lignées fondatrices du phylloxéra (Daktulosphaira vitifoliae Fitch). Huit lignées clonales, élevées sous des conditions contrôlées dans une serre et se reproduisant asexuellement pendant un minimum de 15 générations, ont été examinées et les mutations ont été décelées sous la forme de polymorphismes distinguant l'individu fondateur d'individus des générations suivantes. La variation génétique a été détectée au sein de toutes les lignées, dès les premières générations. Entre 6 et 15 locus polymorphes (sur un total de 141 locus) ont été détectés au sein des lignées, correspondant à 4,3 % de la variation génétique totale. La présence de contaminants extra-génomiques, tels que de l'ADN viral, bactérien ou chloroplastique ingéré, a été exclue comme source de la variation intraclonale. Le séquençage de 37 bandes polymorphes choisies a confirmé qu'elles provenaient principalement de régions non-codantes du génome du phylloxéra. Les techniques AFLP se sont montrées puissantes pour l'identification d'empreintes reproductibles au sein de lignées clonales.

Document Type: Research article

Publication date: 2007-07-01

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• From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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