Authors: Wang, Chih-Li; Malkus, Arkadiusz; Zuzga, Sabina M.; Chang, Pi-Fang Linda; Cunfer, Barry M.; Arseniuk, Edward; Ueng, Peter P.

Source: Genome, Volume 50, Number 6, June 2007 , pp. 595-609(15)

Publisher: NRC Research Press

Abstract:

Phaeosphaeria species are important causal agents of Stagonospora leaf blotch diseases in cereals. In this study, the nucleotide sequence and deduced polypeptide of the trifunctional histidine biosynthesis gene (his) are used to investigate the phylogenetic relationships and provide molecular identification among cereal Phaeosphaeria species. The full-length sequences of the his gene were obtained by PCR amplification and compared among cereal Phaeosphaeria species. The coding sequence of the his gene in wheat-biotype P. nodorum (PN-w) was 2697 bp. The his genes in barley-biotype P. nodorum (PN-b), two P. avenaria f. sp. triticea isolates (homothallic Pat1 and Pat3), and Phaeosphaeria species from Polish rye and dallis grass were 2694 bp. The his gene in heterothallic isolate Pat2, however, was 2693 bp because the intron had one fewer base. In P. avenaria f. sp. avenaria (Paa), the his gene was only 2670 bp long. The differences in the size of the his gene contributed to the variation in amino acid sequences in the gap region located between the phosphoribosyl-ATP pyrophosphohydrolase and histidinol dehydrogenase sub-domains. Based on nucleotide and deduced amino acid sequences of the his gene, Pat1 was not closely related to either PN-w or the Paa clade. It appears that rates of evolution of the his gene were fast in cereal Phaeosphaeria species. The possible involvement of meiotic recombination in genetic diversity of the his gene in P. nodorum is discussed.

Les espèces du genre Phaeosphaeria sont d'importants agents des taches septoriennes chez les céréales. Dans ce travail, les séquences nucléotidique et peptidique déduite du gène his, lequel code pour une enzyme responsable de trois étapes de la biosynthèse de l'histidine, ont été employées pour étudier les relations phylogénétiques et pour l'identification moléculaire chez les espèces de Phaeosphaeria touchant les céréales. Les séquences complètes de ce gène ont été obtenues par amplification PCR et comparées chez les espèces de Phaeosphaeria touchant les céréales. La séquence codante du gène his chez le biotype du P. nodorum infectant le blé (PN-w) mesurait 2697 pb. Les gènes his chez le biotype du P. nodorum touchant l'orge (PN-b), chez deux isolats du P.avenaria f. sp. triticea (homothallique Pat1 et Pat3), et chez des Phaeosphaeria spp. provenant du seigle polonais et de la digitaire à larges feuilles mesuraient 2694 pb. Le gene his chez l'isolat hétérothallique Pat2 ne comptait que 2693 pb en raison d'une délétion d'une base au sein de l'intron. Chez le P.avenaria f. sp. avenaria (Paa), le gène ne mesurait que 2670 pb. La variation dans la taille des gènes his a contribué à la variation dans les séquences peptidiques au sein de la région séparant les domaines phosphoribosyl-ATP pyrophosphohydrolase et histidinol déshydrogénase. Sur la base des séquences nucléotidique et peptidique du gène his, le Pat1 n'était fortement apparenté ni au clade PN-w ni au clade Paa. Il semblerait que les taux d'évolution du gène his était rapide chez les Phaeosphaeria infectant les céréales. Le rôle possible de la recombinaison méiotique dans la génération de la diversité génétique au sein du gène his chez le P. nodorum est discuté.

Document Type: Research article

Publication date: 2007-06-01

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• From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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