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The mitochondrial genome structure of the clouded leopard (Neofelis nebulosa)

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The complete 16 844 bp mitochondrial genome of Neofelis nebulosa has been sequenced and compared with the complete mitochondrial genomes of Felis catus and the Acinonyx jubatus. The base composition of the mitochondrial genome of N. nebulosa is as follows: A, 5343 bp (31.7%); C, 4441 bp (26.4%); G, 2491 bp (14.8%); T, 4569bp (27.1%). The genome complement and the gene order of this mitochondrial genome was found to be typical of those reported for other mammals. Several unusual features of this genome, however, were found. First, in protein-coding regions, AT bias in the genome was not prevalent in the third position of codons, as it is in most other mammals, but was found in the second position of codons. Second, in tRNA regions, tRNASer (AGY), which lacked the “DHU” arm, could not be folded into the typical cloverleaf-shaped structure. Third, in the control region, no repetitive sequences (RS)-2 were found. However, RS-2 repetitive motifs usually occurr in the control regions of most great cats. In addition, 4 variable sites were found in CSB-3 of the control region. Fourth, AT content in the control region of the mtDNA from the clouded leopard was lower than it is in other regions.

Le génome mitochondrial complet (16 844 pb) du Neofelis nebulosa a été séquencé et comparé avec les génomes mitochondriaux complets du Felis catus et de l’Acinomyx jubatus. La composition nucléotidique du génome mitochondrial du Neofelis nebulosa est comme suit: A, 5 343 pb (31,7 %); C, 4 441 pb (26,4 %); G, 2 491 pb (14,8 %); T, 4569 pb (27,1 %). Le complément génomique et l’ordre des gènes au sein de ce génome mitochondrial est typique de celui rapporté pour les autres mammifères. Plusieurs caractéristiques particulières de ce génome ont cependant été trouvées. D’abord, au sein des régions codant pour des protéines, le biais AT n’était pas prédominant à la troisième position des codons (comme cela est le cas chez la plupart des autres mammifères), mais plutôt à la seconde position. Deuxièmement, dans les régions des ARNt, l’ARNtSer (AGY) auquel il manquait le bras ‘gDHU’ ne pouvait pas se replier pour former la structure en forme de feuille de trèfle typique. Troisièmement, dans la région de contrôle, aucune séquence répétée RS-2 n’a été observée alors que celles-ci sont habituellement retrouvées dans les régions de contrôle chez la plupart des grands félins. De plus, quatre sites variables ont été notés au sein de CSB-3 dans la région de contrôle. Quatrièmement, le contenu en AT au sein de la région de contrôle de l’ADNmt de la panthère nébuleuse était plus faible que dans les autres régions.

Document Type: Research Article

Publication date: February 1, 2007

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  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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