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Alteration of chromosome behavior and synchronization of parental chromosomes after successive generations in Brassica napus × Orychophragmus violaceus hybrids

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Abstract:

In an earlier study, the progenies of intergeneric hybrids Brassica napus (2n = 38) × Orychophragmus violaceus (2n = 24) were investigated in successive generations (F1–F4) for the cytological phenomenon of parental genome separation during mitotic and meiotic division. In the present study, inbred lines (F5–F8) derived from 1 such hybrid were characterized for morphology, chromosome pairing behaviour, and genome composition. One F5 plant (2n = 31) with slightly yellow petals and 12:19 and 15:16 segregation ratios in its pollen mother cells (PMCs) produced F6 plants with distinct morphological characteristics and wide variations in fertility and chromosome numbers (2n = 25–38). F7 and F8 lines with distinctive morphology and wide ranges in chromsome numbers were established. In PMCs of F7 plants from 4 F6 plants, 0–12 labelled chromosomes from O. violaceus, which predominantly appeared as bivalents, were identified by genomic in situ hybridization. They behaved synchronously with B. napus chromosomes during meiotic division. The results provide molecular cytogenetic evidence of the inclusion of O. violaceus chromosomes in the original hybrids and the cytology in the hybrids documented earlier. They also show that chromosome behaviour was altered and the parental chromosomes became synchronized after successive generations.

Les progénitures d’hybrides intergénériques Brassica napus (2n= 38)× Orychophragmus violaceus (2n= 24) ont été étudiées précédemment au sein des premières générations (F1-F4) pour examiner le phénomène de séparation des génomes parentaux au cours des divisions mitotiques et méiotiques. Dans le présent travail, les lignées autofécondées (F5–F8) issues d’un de ces hybrides ont été caractérisées pour leur morphologie, l’appariement des chromosomes et leur composition génomique. Une plante F5 (2n= 31) montrant des pétales jaunes et des ségrégations 12:19 et 15:16 chez les microsporocytes (PMC) a produit des plantes F6 présentant des caractères morphologiques distincts et de grandes variations quant à la fécondité et au nombre chromosomique (2n= 25–38). Des lignées F7 et F8 affichant des morphologies et des nombres chromosomiques très différents ont été établies. Chez les PMC de plantes F7 issues de quatre plantes F6, entre 0 et 12 chromosomes marqués de l’O.violaceus, sous la forme prédominante de bivalents, ont été identifiés par hybridation génomique in situ. Ceux-ci se comportaient de manière synchrone par rapport aux chromosomes du B. napus au cours des divisions méiotiques. Les résultats fournis par les analyses cytogénétiques moléculaires impliquent l’inclusion de chromosomes de l’O.violaceus au sein des hybrides d’origine et expliquent les observations cytologiques faites antérieurement sur les hybrides. Ces travaux montrent également que le comportement des chromosomes a été altéré et que les chromosomes parentaux se sont synchronisés au terme de quelques générations.

Document Type: Research Article

Publication date: 2007-02-01

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  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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