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Linkage maps for Arabidopsis lyrata subsp. lyrata and Arabidopsis lyrata subsp. petraea combining anonymous and Arabidopsis thaliana–derived markers

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Abstract:

Arabidopsis lyrata, a close relative of the model plant Arabidopsis thaliana, is 1 of a few plant species for which the genome is to be entirely sequenced, which promises to yield important insights into genome evolution. Only 2 sparse linkage maps have been published, and these were based solely on markers derived from the A. thaliana genome. Because the genome of A. lyrata is practically twice as large as that of A. thaliana, the extent of map coverage of the A. lyrata genome remains uncertain. In this study, a 2-way pseudo-testcross strategy was used to construct genetic linkage maps of A. lyrata subsp. petraea and A. lyrata subsp. lyrata, using simple sequence repeat (SSR) and cleaved amplified polymorphic sequence (CAPS) markers from the A. thaliana genome, and anonymous amplified fragment length polymorphism (AFLP) markers that could potentially uncover regions unique to the A. lyrata genome. The SSR and CAPS markers largely confirmed the relationships between linkage groups in A. lyrata and A. thaliana. AFLP markers slightly increased the coverage of the A. lyrata maps, but mostly increased marker density on the linkage groups. We noted a much lower level of polymorphism and a greater segregation distortion in A. lyrata subsp. lyrata markers. The implications of these findings for the sequencing of the A. lyrata genome are discussed.

Arabidopsis lyrata, un proche parent de la plante modèle A. thaliana, est une des rares espèces dont le génome sera entièrement séquencé car il promet d’apporter des connaissances importantes sur l’évolution des génomes. Seules deux cartes de liaison de faible densité ont été publiées et celles-ci sont basées uniquement sur des marqueurs dérivés du génome d’A. thaliana. Puisque le génome d'A. lyrata est pratiquement deux fois plus grand que celui d'A. thaliana, l’étendue de la couverture de la carte du génome d'A. lyrata demeure incertaine. Dans cette étude, la stratégie d’un pseudo-testcross réciproque a été employée afin de construire les cartes de liaisons génétiques d'A. lyrata subsp. petraea et d'A. lyrata subsp. lyrata en utilisant des marqueurs SSR et CAPS du génome d'A. thaliana et des marqueurs AFLP anonymes qui pourraient potentiellement révéler des régions uniques au génome d'A. lyrata. Les marqueurs SSR et CAPS ont confirmé en grande partie les relations entre les groupes de liaison d'A. lyrata et d'A. thaliana. Les marqueurs AFLP ont accru légèrement la couverture des cartes d'A. lyrata, mais ont surtout permis d’augmenter la densité de marqueurs sur les groupes de liaison. Nous avons noté un niveau plus bas de polymorphisme et une plus grande distorsion de ségrégation chez les marqueurs d’A. lyrata subsp. lyrata. Les implications de ces résultats pour le séquençage du génome d'A. lyrata sont discutées.

Document Type: Research Article

Publication date: February 1, 2007

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  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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