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Retrotransposon-based S-SAP as a platform for the analysis of genetic variation and linkage in globe artichoke

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Abstract:

A high copy number of retrotransposon sequences are present and widely dispersed in plant genomes. Their activity generates a considerable degree of sequence polymorphism. Here, we report the cloning of CYRE-5, a long-terminal repeat carrying retrotransposon-like sequence in Cynara cardunculus L., and its exploitation to develop a DNA fingerprinting assay across 22 accessions, including both cultivated (globe artichoke and cultivated cardoon) and wild (wild cardoon) types. The effectiveness of the sequence-specific amplified polymorphism (S-SAP) platform is compared with that of amplified fragment length polymorphism (AFLP). A genetic linkage analysis, based on a hybrid population between 2 globe artichoke varietal types, resulted in the inclusion of 29 S-SAP loci in the core genetic map, confirming their dispersed distribution across the globe artichoke genome.

Les rétrotransposons sont présents en un grand nombre de copies et sont largement dispersés dans les génomes des plantes. Leur activité génère une quantité appréciable de polymorphisme de séquence. Les auteurs rapportent le clonage de CYRE-5, une séquence semblable à des rétrotransposons à longues terminaisons répétées, chez le Cynara cardunculus L. Ils décrivent son utilisation pour mettre au point une méthode de production d’empreintes génétiques employée sur 22 accessions comprenant à la fois des types cultivés (artichaut et cardon) et sauvages (cardon sauvage). L’efficacité de la technique S-SAP («sequence-specific amplified polymorphism») a été comparée à celle de la technique AFLP. Une analyse de liaison génétique, faisant appel à une population issue du croisement de deux cultivars d’artichaut, a permis d’inclure 29 locus S-SAP sur la carte génétique, ce qui confirmait leur dispersion dans le génome de l’artichaut.

Document Type: Research Article

Publication date: 2006-09-01

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  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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