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Physical mapping of the blue-grained gene(s) from Thinopyrum ponticum by GISH and FISH in a set of translocation lines with different seed colors in wheat

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Abstract:

The original blue-grained wheat, Blue 58, was a substitution line derived from hybridization between common wheat (Triticum aestivum L., 2n = 6x = 42, ABD) and tall wheatgrass (Thinopyrum ponticum Liu & Wang = Agropyron elongatum, 2n = 10x = 70, StStEeEbEx), in which one pair of 4D chromosomes was replaced by a pair of alien 4Ag chromosomes (unknown group 4 chromosome from A. ponticum). Blue aleurone might be a useful cytological marker in chromosome engineering and wheat breeding. Cytogenetic analysis showed that blue aleurone was controlled by chromosome 4Ag. GISH analysis proved that the 4Ag was a recombination chromosome; its centromeric and pericentromeric regions were from an E-genome chromosome, but the distal regions of its two arms were from an St-genome chromosome. On its short arm, there was a major pAs1 hybridization band, which was very close to the centromere. GISH and FISH analysis in a set of translocation lines with different seed colors revealed that the gene(s) controlling the blue pigment was located on the long arm of 4Ag. It was physically mapped to the 0.71–0.80 regions (distance measured from the centromere of 4Ag). The blue color is a consequence of dosage of this small chromosome region derived from the St genome. We speculate that the blue-grained gene(s) could activate the anthocyanin biosynthetic pathway of wheat.

Le premier blé à grains bleus, Blue 58, était une lignée de substitution dérivée d’un croisement entre le blé tendre (Triticum aestivum L., 2n = 6x = 42, ABD) et le chiendent allongé (Thinopyrum ponticum Liu & Wang = Agropyron elongatum, 2n = 10x = 70, StStEcEbEx) chez laquelle une paire de chromosomes 4D avait été remplacée par une paire de chromosomes étrangers 4Ag (un chromosome du groupe 4 de l’A. ponticum). L’aleurone bleue pourrait s’avérer un marqueur cytologique utile lors de travaux d’ingénierie chromosomique chez le blé. Une analyse cytogénétique a montré que l’aleurone bleue était déterminée par le chromosome 4Ag. Une analyse GISH a démontré que le chromosome 4Ag est recombiné; les régions centromériques et péricentromériques proviennent d’un chromosome d’un génome E alors que les régions distales des 2 bras proviennent d’un chromosome du génome St. Sur le bras court, un fort signal d’hybridation avec la sonde pAs1 a été noté très proche du centromère. Des analyses GISH et FISH au sein d’un jeu de lignées de translocation ayant diverses couleurs du grain a révélé que le ou les gènes conférant une coloration bleue sont situés sur le bras long de 4Ag. Il a été physiquement positionné au sein de la région 0,71 à 0,80 à partir du centromère de 4Ag. La couleur bleue découle d’un effet de dose de cette petite région dérivée du génome St. Les auteurs spéculent que les gènes conférant une couleur bleue aux grains pourrait activer le sentier de synthèse des anthocyanes chez le blé.

Document Type: Research Article

Publication date: September 1, 2006

More about this publication?
  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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