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Current status and the future of fluorescence in situ hybridization (FISH) in plant genome research

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Abstract:

Fluorescence in situ hybridization (FISH), which allows direct mapping of DNA sequences on chromosomes, has become the most important technique in plant molecular cytogenetics research. Repetitive DNA sequence can generate unique FISH patterns on individual chromosomes for karyotyping and phylogenetic analysis. FISH on meiotic pachytene chromosomes coupled with digital imaging systems has become an efficient method to develop physical maps in plant species. FISH on extended DNA fibers provides a high-resolution mapping approach to analyze large DNA molecules and to characterize large genomic loci. FISH-based physical mapping provides a valuable complementary approach in genome sequencing and map-based cloning research. We expect that FISH will continue to play an important role in relating DNA sequence information to chromosome biology. FISH coupled with immunoassays will be increasingly used to study features of chromatin at the cytological level that control expression and regulation of genes.

L’hybridation in situ en fluorescence (FISH), laquelle permet de positionner directement une séquence d’ADN sur un chromosome, est devenue la technique la plus importante en cytogénétique moléculaire chez les plantes. Des séquences répétitives d’ADN peuvent générer des bandes FISH sur des chromosomes individuels pour fins de caryotypage et d’analyse phylogénétique. Employée sur des chromosomes méiotiques au stade pachytène et couplée à l’emploi de systèmes d’imagerie numérique, l’analyse FISH est devenue une méthode efficace pour développer des cartes physiques chez les espèces végétales. Appliquée à des fibres étirées d’ADN, l’analyse FISH fournit une approche à haute résolution pour analyser de grandes molécules d’ADN et pour caractériser de grands locus génomiques. La cartographie physique par FISH se veut une approche complémentaire précieuse pour le séquençage des génomes et le clonage positionnel. Les auteurs prédisent que la FISH continuera de jouer un rôle important permettant de relier l’information sur des séquences d’ADN et la biologie des chromosomes. La FISH, couplée à des méthodes immunologiques, sera de plus en plus employée pour étudier les caractéristiques de la chromatine au niveau cytologique, lesquelles contribuent à l’expression et à la régulation des gènes.

Document Type: Research Article

Publication date: September 1, 2006

More about this publication?
  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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